Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8022, USA.
J Infect Dis. 2013 Oct 1;208(7):1161-9. doi: 10.1093/infdis/jit284. Epub 2013 Jun 24.
Malaria, a mosquito-borne disease caused by Plasmodium species, causes substantial morbidity and mortality throughout the world. Plasmodium sporozoites mature in oocysts formed in the mosquito gut wall and then invade the salivary glands, where they remain until transmitted to the vertebrate host during a mosquito bite. The Plasmodium circumsporozoite protein (CSP) binds to salivary glands and plays a role in the invasion of this organ by sporozoites. We identified an Anopheles salivary gland protein, named CSP-binding protein (CSPBP), that interacts with CSP. Downregulation of CSPBP in mosquito salivary glands inhibited invasion by Plasmodium organisms. In vivo bioassays showed that mosquitoes that were fed blood with CSPBP antibody displayed a 25% and 90% reduction in the parasite load in infected salivary glands 14 and 18 days after the blood meal, respectively. These results suggest that CSPBP is important for the infection of the mosquito salivary gland by Plasmodium organisms and that blocking CSPBP can interfere with the Plasmodium life cycle.
疟疾是一种由疟原虫属引起的蚊媒疾病，在全球范围内造成了大量的发病率和死亡率。疟原虫子孢子在蚊肠道壁形成的卵囊中成熟，然后侵入唾液腺，在那里它们一直存在，直到在蚊子叮咬时传播到脊椎动物宿主。疟原虫环子孢子蛋白（CSP）与唾液腺结合，并在子孢子侵入该器官中发挥作用。我们鉴定了一种名为 CSP 结合蛋白（CSPBP）的疟蚊唾液腺蛋白，它与 CSP 相互作用。在蚊子唾液腺中下调 CSPBP 抑制了疟原虫的入侵。体内生物测定显示，在吸血后 14 天和 18 天，用 CSPBP 抗体喂养的蚊子，感染唾液腺中的寄生虫负荷分别减少了 25%和 90%。这些结果表明 CSPBP 对疟原虫感染蚊子唾液腺很重要，阻断 CSPBP 可以干扰疟原虫的生命周期。
