Okuno Y, Tanaka K, Baba K, Maeda A, Kunita N, Ueda S
Department of Preventive Medicine, Osaka University, Suita, Japan.
J Clin Microbiol. 1990 Jun;28(6):1308-13. doi: 10.1128/jcm.28.6.1308-1313.1990.
A rapid neutralization test for influenza A and B viruses was developed. In this method, a 96-well tissue culture plate was used for the preparation of cell monolayers and the peroxidase-antiperoxidase staining technique was used for the visualization of foci infected with these viruses. In the presence of trypsin and tragacanth gum, clear foci developed 1 day after infection. A linear relationship between virus dilutions and numbers of foci was observed. When neutralizing antibodies in some test sera were assayed, a good correlation was observed between the titers obtained by the focus method and those obtained by the ordinary plaque method. In addition, many serum specimens were investigated by the neutralization test, and it was demonstrated that the test is useful for serological studies of influenza.
开发了一种针对甲型和乙型流感病毒的快速中和试验。在该方法中，使用96孔组织培养板制备细胞单层，并使用过氧化物酶-抗过氧化物酶染色技术来观察感染这些病毒的病灶。在胰蛋白酶和黄芪胶存在的情况下，感染后1天出现清晰的病灶。观察到病毒稀释度与病灶数量之间存在线性关系。当检测一些试验血清中的中和抗体时，通过病灶法获得的滴度与通过普通蚀斑法获得的滴度之间观察到良好的相关性。此外，通过中和试验对许多血清标本进行了研究，结果表明该试验对流感的血清学研究有用。
