流感病毒中和酶免疫测定法
Neutralization enzyme immunoassay for influenza virus.
作者信息
Benne C A, Harmsen M, De Jong J C, Kraaijeveld C A
机构信息
Eijkman-Winkler Laboratory of Medical Microbiology, University Hospital Utrecht, The Netherlands.
出版信息
J Clin Microbiol. 1994 Apr;32(4):987-90. doi: 10.1128/jcm.32.4.987-990.1994.
Abstract
A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 cells in microtiter plates. After 24 h, the replicated virus could be demonstrated with a virus-specific enzyme-labeled monoclonal antibody. Preincubation of the influenza virus with convalescent-phase sera of patients infected with influenza A (H3N2) virus resulted 1 day later in decreased absorbance values that could be used for calculation of neutralization titers. From use of paired serum samples from 10 patients with a history of flu-like symptoms, the results obtained with N-EIA correlated well (r = 0.83) with those of the standard hemagglutination inhibition test.
摘要
开发了一种中和酶免疫测定法（N-EIA），用于检测感染甲型流感（H3N2）病毒患者血清中抗体滴度的升高。在这种N-EIA中，将选定的甲型流感（H3N2）病毒株添加到微量滴定板中的LLC-MK2细胞单层中。24小时后，用病毒特异性酶标记单克隆抗体可检测到复制的病毒。甲型流感病毒与感染甲型流感（H3N2）病毒患者的恢复期血清预孵育1天后，吸光度值降低，可用于计算中和滴度。使用10例有流感样症状病史患者的配对血清样本，N-EIA获得的结果与标准血凝抑制试验的结果相关性良好（r = 0.83）。
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