INRS-Institut Armand-Frappier, Laval, Québec, Canada.
J Virol. 2013 Sep;87(17):9928-32. doi: 10.1128/JVI.01259-13. Epub 2013 Jun 26.
The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.
白纹伊蚊浓核病毒(AalDNV) brevidensovirus 的转录图谱通过 Northern 印迹、快速扩增 cDNA 末端 (RACE) 分析和 RNase 保护试验确定。AalDNV 产生 3,359(NS1)、3,345(NS2)和 1,246(VP)个核苷酸的 mRNA。两个重叠的 P7/7.4 NS 启动子使用靠近的交替转录起始位点,位于 NS1 起始密码子的两侧。所有 NS mRNA 均与 VP mRNA 终止。所有启动子均通过荧光素酶测定进行了探索,在昆虫和人细胞系中均具有功能。