多不饱和磷脂乙醇胺的弹性性质影响视紫红质的功能。
Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function.
机构信息
Laboratory of Membrane Biochemistry and Biophysics, NIAAA, NIH, Bethesda, MD 20892, USA.
出版信息
Faraday Discuss. 2013;161:383-95; discussion 419-59. doi: 10.1039/c2fd20095c.
Abstract
Membranes with a high content of polyunsaturated phosphatidylethanolamines (PE) facilitate formation of metarhodopsin-II (M(II)), the photointermediate of bovine rhodopsin that activates the G protein transducin. We determined whether M(II)-formation is quantitatively linked to the elastic properties of PEs. Curvature elasticity of monolayers of the polyunsaturated lipids 18 : 0-22 : 6(n - 3)PE, 18 : 0-22 : 5(n)- 6PE and the model lipid 18 : 1(n - 9)-18 : 1,(n- 9)PE were investigated in the inverse hexagonal phase. All three lipids form lipid monolayers with rather low spontaneous radii of curvature of 26-28 angstroms. In membranes, all three PEs generate high negative curvature elastic stress that shifts the equilibrium of MI(I)/M(II) photointermediates of rhodopsin towards M(II) formation.
摘要
具有高含量多不饱和磷脂酰乙醇胺 (PE) 的膜有利于形成视紫红质的中间产物视黄醛 II (M(II))，这是牛视紫红质的光中间产物，可激活 G 蛋白转导蛋白。我们确定了 M(II)形成是否与 PE 的弹性性质定量相关。我们在反六方相下研究了多不饱和脂质 18:0-22:6(n-3)PE、18:0-22:5(n)-6PE 和模型脂质 18:1(n-9)-18:1(n-9)PE 的单层的曲率弹性。这三种脂质都形成具有相当低的自发曲率半径为 26-28 埃的脂质单层。在膜中，这三种 PE 都会产生高负曲率弹性应力，使视紫红质的 MI(I)/M(II)光中间产物的平衡向 M(II)形成方向移动。
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