Breddin K, Grun H, Krzywanek H J, Schremmer W P
Klin Wochenschr. 1975 Jan 15;53(2):81-9. doi: 10.1007/BF01482713.
A new measuring device for the estimation of the "spontaneous" aggregating activity of thrombocytes has been developed. In this photometric platelet aggregation test (PAT III) a small amount (0.6 ml) of platelet-rich plasma (PRP) is being rotated in a disc-shaped cuvette at 20 rpm, at 37% C. Changes in optical density of PRP which are induced by the formation of platelet aggregates are continuously registered using a chart recorder. The decisive trigger mechanism for aggregation is an increase of plasma pH in the rotating sample which is caused by evaporation of CO2. The results of the test depend on the platelet count in PRP. Aggregation curves are misrepresented by admixture of erythrocytes and lipid turbidity. The tendency of platelets to aggregate increases within 60-90 min following blood sampling. During this period the time interval to the onset of aggregation(Tr) is shortening, and the maximum aggregation speed (alpha2) is increasing. The spontaneously enhanced aggregation tendency of thrombocytes may be reliably measured from 60 min after drawing the blood. The reason for these time-dependent changes which are also demonstrable in ADP-collagen-or epinephrine-induced aggregation is probably the primary shape change of platelets, which occurs after blood drawing and makes them "stickly" and aggregable. PAT III was developed for the detection of enhanced platelet aggregation, indicating a risk of thrombosis and thromboembolic omplications. The new measuring device has been designed as "universal" aggregometer. Additional equipment is available for the registration of ADP-collagen-or epinephrine-induced aggregation similar to Born's and O'Brien's methods. The device may be mounted easily on an Eppendorf photometer without further modifications.
已开发出一种用于评估血小板“自发”聚集活性的新型测量装置。在这种光度血小板聚集试验（PAT III）中，少量（0.6毫升）富含血小板的血浆（PRP）在盘形比色皿中于37℃以20转/分钟的速度旋转。由血小板聚集体形成引起的PRP光密度变化通过图表记录仪持续记录。聚集的决定性触发机制是旋转样品中由于二氧化碳蒸发导致的血浆pH值升高。测试结果取决于PRP中的血小板计数。红细胞的混入和脂质浑浊会使聚集曲线出现偏差。采血后60 - 90分钟内血小板聚集的趋势增加。在此期间，聚集开始的时间间隔（Tr）缩短，最大聚集速度（α2）增加。血小板自发增强的聚集趋势可在采血后60分钟起可靠测量。这些与时间相关的变化在ADP - 胶原或肾上腺素诱导的聚集中也可观察到，其原因可能是采血后血小板发生的初始形态变化，使其变得“黏附性强”且易于聚集。PAT III是为检测增强的血小板聚集而开发的，这表明存在血栓形成和血栓栓塞并发症的风险。这种新型测量装置被设计为“通用”凝集仪。还有额外的设备可用于记录类似于博恩（Born）和奥布赖恩（O'Brien）方法的ADP - 胶原或肾上腺素诱导的聚集。该装置可轻松安装在艾本德（Eppendorf）光度计上，无需进一步改装。
