Suman Ethel, Varghese Benji, Joseph Neethu, Nisha Kumari, Kotian M Shashidhar
Associate Professor, Department of Microbiology Kasturba Medical College , Mangalore, Manipal University, Karnataka State, India .
J Clin Diagn Res. 2013 May;7(5):849-52. doi: 10.7860/JCDR/2013/5118.2956. Epub 2013 May 1.
The presence of bacteria in the form of biofilms poses a problem in the fluid pathways of haemodialysis plants and procedures which are aimed to detach and neutralize biofilms are necessary to improve the patient safety and the quality of the healthcare. The present study was therefore aimed at isolating the organisms which colonized dialysis water systems as biofilms, as well as to study the effect of the sub inhibitory concentrations of chlorine on the biofilms which were produced by these isolates.
Swabs were used to collect the biofilms which were produced on the internal surface of the dialysis tubing from the dialysis units. This study was conducted at the Department of Microbiology, Kasturba Medical College (KMC), Mangalore, India. The cultures were performed on MacConkey's agar and blood agar. The organisms which were isolated were identified and antibiotic sensitivity tests were performed. The biofilm production was done by the microtitre plate method of O'Toole and Kolter. The biofilm production was also studied in the presence of sub inhibitory concentrations of chlorine.
Acinetobacter spp and Pseudomonas aeruginosa were the two predominant organisms which colonized the dialysis water systems as biofilms. The sub inhibitory concentrations of chlorine did not bring about any decrease in the biofilm production by the isolates. On the contrary, there was an increase in the biofilm production.
Our study highlighted the importance of using appropriate methods to improve the quality of the water in dialysis units. This in turn, may help in reducing the biofilm formation in the water systems of dialysis units and thus, contribute to the prevention of hospital acquired infections in the patients who need haemodialysis.
生物膜形式的细菌存在于血液透析设备的流体通路中，这是一个问题，旨在分离和中和生物膜的程序对于提高患者安全性和医疗质量是必要的。因此，本研究旨在分离以生物膜形式定殖于透析水系统的微生物，并研究亚抑制浓度的氯对这些分离株产生的生物膜的影响。
用拭子收集透析单元中透析管内表面产生的生物膜。本研究在印度芒格洛尔卡斯图尔巴医学院（KMC）微生物学系进行。在麦康凯琼脂和血琼脂上进行培养。对分离出的微生物进行鉴定并进行药敏试验。生物膜的产生采用奥图尔和科尔特的微量滴定板法。还研究了在亚抑制浓度的氯存在下生物膜的产生情况。
不动杆菌属和铜绿假单胞菌是作为生物膜定殖于透析水系统的两种主要微生物。氯的亚抑制浓度并未使分离株的生物膜产生量有任何减少。相反，生物膜产生量有所增加。
我们的研究强调了使用适当方法提高透析单元水质的重要性。这反过来可能有助于减少透析单元水系统中的生物膜形成，从而有助于预防需要血液透析的患者发生医院获得性感染。
