Tomusiak Anna, Heczko Piotr Bogumił, Janeczko Jarosław, Adamski Paweł, Pilarczyk-Zurek Magdalena, Strus Magdalena
Katedra Mikrobiologii Collegium Medicum Uniwersytetu Jagiellońskiego, Kraków, Poska.
Ginekol Pol. 2013 May;84(5):352-8. doi: 10.17772/gp/1588.
The objective of the study was to investigate the detection rates of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Gardnerella vaginalis, Escherichia coli, Streptococcus agalactiae and Enterococcus faecalis, showing no clinical signs of an ongoing, acute inflammatory state of the vagina and/or the cenrvix, in fertile and infertile women.
The study encompassed 161 women, including 101 women treated for infertility and 60 fertile women who had already given birth to healthy children. The material for the presence of C. trachomatis, N. gonorrhoeae, M. genitalium, M. hominis and U. urealyticum was collected from the cervical canal and analyzed by PCR. Furthermore, BD ProbeTec ET system was used to detect C. trachomatis infection. Vaginal swabs were collected for classification of bacterial vaginosis and aerobic vaginitis and assessed according to the Nugent score, as well as by traditional culture methods.
U. urealyticum was identified in 9% of the infertile women and in 8% of controls. Presence of M. hominis was demonstrated only in the former (4%) and C. trachomatis only in latter (3%). N. gonorrhoeae and M. genitalium were not found in any of the examined women. The frequency of aerobic vaginitis in both groups was estimated at 12%. There were 7% bacterial vaginosis cases in the study group, and none in the control group (p=0.0096).
Despite having no symptoms of an ongoing acute inflammation of the reproductive tract, many women may experience permanent or periodic shifts of equilibrium of the vaginal and/or cervical microflora. BV develops more frequently in infertile patients when compared to the fertile women.
本研究的目的是调查沙眼衣原体、淋病奈瑟菌、生殖支原体、人型支原体、解脲脲原体、阴道加德纳菌、大肠埃希菌、无乳链球菌和粪肠球菌在无阴道和/或宫颈持续急性炎症临床症状的育龄和不孕妇女中的检出率。
该研究纳入了161名女性，其中包括101名接受不孕症治疗的女性和60名已生育健康子女的育龄女性。从宫颈管收集用于检测沙眼衣原体、淋病奈瑟菌、生殖支原体、人型支原体和解脲脲原体的材料，并通过聚合酶链反应（PCR）进行分析。此外，使用BD ProbeTec ET系统检测沙眼衣原体感染。收集阴道拭子用于细菌性阴道病和需氧菌性阴道炎的分类，并根据 Nugent评分以及传统培养方法进行评估。
解脲脲原体在9%的不孕妇女和8%的对照组中被鉴定出来。人型支原体仅在前一组（4%）中被检测到，而沙眼衣原体仅在后一组（3%）中被检测到。在所检查的女性中均未发现淋病奈瑟菌和生殖支原体。两组中需氧菌性阴道炎的发生率估计为12%。研究组中有7%的细菌性阴道病病例，而对照组中无一例（p = 0.0096）。
尽管许多女性没有生殖道持续急性炎症的症状，但她们的阴道和/或宫颈微生物群平衡可能会出现永久性或周期性变化。与育龄女性相比，细菌性阴道病在不孕患者中更频繁发生。
