Tsuge Yota, Hasunuma Tomohisa, Kondo Akihiko
Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
J Ind Microbiol Biotechnol. 2015 Mar;42(3):375-89. doi: 10.1007/s10295-014-1538-9. Epub 2014 Nov 26.
Recent increasing attention to environmental issues and the shortage of oil resources have spurred political and industrial interest in the development of environmental friendly and cost-effective processes for the production of bio-based chemicals from renewable resources. Thus, microbial production of commercially important chemicals is viewed as a desirable way to replace current petrochemical production. Corynebacterium glutamicum, a Gram-positive soil bacterium, is one of the most important industrial microorganisms as a platform for the production of various amino acids. Recent research has explored the use of C. glutamicum as a potential cell factory for producing organic acids such as lactate and succinate, both of which are commercially important bulk chemicals. Here, we summarize current understanding in this field and recent metabolic engineering efforts to develop C. glutamicum strains that efficiently produce L- and D-lactate, and succinate from renewable resources.
近期，对环境问题的日益关注以及石油资源的短缺激发了政治界和工业界对开发从可再生资源生产生物基化学品的环境友好且具有成本效益的工艺的兴趣。因此，微生物生产具有商业重要性的化学品被视为取代当前石化生产的理想方式。谷氨酸棒杆菌是一种革兰氏阳性土壤细菌，作为生产各种氨基酸的平台，它是最重要的工业微生物之一。最近的研究探索了将谷氨酸棒杆菌用作潜在的细胞工厂来生产诸如乳酸和琥珀酸之类的有机酸，这两种都是具有商业重要性的大宗化学品。在此，我们总结了该领域的当前认识以及最近为开发能从可再生资源高效生产L-和D-乳酸以及琥珀酸的谷氨酸棒杆菌菌株所做的代谢工程努力。
