Therapeutics Clinical Research, San Diego, CA, United States.
J Dermatol Sci. 2013 Nov;72(2):87-92. doi: 10.1016/j.jdermsci.2013.06.001. Epub 2013 Jun 12.
Psoriasis shares many features with wound healing, a process that involves switching keratinocytes from growth to differentiation. Ca2+ is known to regulate this process. The N-methyl-d-aspartate receptor (NMDAR), an ionotropic glutamate receptor found on keratinocytes, is expressed abnormally in psoriasis in vivo.
The goals of this study are to determine whether the rate of healing in the skin of psoriatic individuals differs from that observed in normal skin and whether the keratinocyte hyperproliferation found in psoriasis correlates with expression of specific NMDAR subunits.
Three mm punch biopsies were performed on the skin of normal, as well as, involved and uninvolved skin of subjects with psoriasis. On day 0, as well as, on day 6 after the biopsy, photographs were taken and the size of the wounds determined using ImageJ. Using immunohistochemistry, the biopsy material was stained for NMDAR and its subunits.
Involved and uninvolved skin of individuals with psoriasis shows significantly more rapid healing than normal. The NR2C subunit of NMDAR is down-regulated in the basal cell layer of involved and uninvolved epidermis of psoriatic subjects compared to controls. By contrast, cells in the basal cell layer of the uninvolved epidermis showed a significantly greater percent strong staining for NR2D compared to those cells in normal epidermis.
Wound healing is significantly accelerated in psoriasis compared to normal. Immunohistochemistry showed that the relative intensity of strong immunostaining for subunits of the NMDAR is altered in the basal cell layer in psoriatic skin compared to normal controls. We suggest that these alterations may contribute to the increased rate of wound healing in psoriasis.
银屑病与伤口愈合有许多共同特征，这是一个涉及角质细胞从生长到分化转变的过程。已知钙离子调节这个过程。N-甲基-D-天冬氨酸受体（NMDAR）是一种存在于角质细胞上的离子型谷氨酸受体，在体内银屑病中表达异常。
本研究的目的是确定银屑病患者皮肤的愈合速度是否与正常皮肤观察到的愈合速度不同，以及银屑病中发现的角质细胞过度增殖是否与特定 NMDAR 亚基的表达相关。
对正常皮肤以及银屑病患者受累和未受累皮肤进行 3mm 打孔活检。在第 0 天和活检后第 6 天，拍摄照片并使用 ImageJ 确定伤口大小。使用免疫组织化学方法，对活检材料进行 NMDAR 及其亚基染色。
银屑病患者受累和未受累皮肤的愈合速度明显快于正常皮肤。与对照组相比，NR2C 亚基在银屑病受累和未受累表皮的基底层下调。相比之下，未受累表皮基底层的细胞对 NR2D 的强烈染色百分比明显高于正常表皮细胞。
与正常皮肤相比，银屑病的伤口愈合明显加快。免疫组织化学显示，与正常对照组相比，银屑病皮肤基底层 NMDAR 亚基的强烈免疫染色相对强度发生改变。我们认为这些改变可能导致银屑病中伤口愈合速度加快。
