基质辅助激光解吸电离飞行时间质谱可准确区分马萨利分枝杆菌（脓肿分枝杆菌亚种博莱氏菌）和脓肿分枝杆菌（严格意义上的）。
Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (sensu stricto).
机构信息
Department of Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan.
出版信息
J Clin Microbiol. 2013 Sep;51(9):3113-6. doi: 10.1128/JCM.01239-13. Epub 2013 Jul 3.
Abstract
Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
摘要
在通过 erm(41)PCR 和 rpoB 及 23S rRNA 基因测序分析鉴定的 36 株 Mycobacterium masilliense 和 22 株 M. abscessus 分离株中，Bruker Biotyper 基质辅助激光解吸电离飞行时间质谱产生的光谱聚类分析可 100%准确地区分这两个亚种。
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