基质辅助激光解吸电离飞行时间质谱能够准确地区分达卡气单胞菌与嗜水气单胞菌、豚鼠气单胞菌和维氏气单胞菌。
Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate Aeromonas dhakensis from A. hydrophila, A. caviae, and A. veronii.
作者信息
Chen Po-Lin, Lee Tai-Fen, Wu Chi-Jung, Teng Shih-Hua, Teng Lee-Jene, Ko Wen-Chien, Hsueh Po-Ren
机构信息
Graduate Institute of Clinical Medicine, National Cheng Kung University, College of Medicine, Tainan, Taiwan Department of Internal Medicine, National Cheng Kung University, College of Medicine and Hospital, Tainan, Taiwan.
Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan.
出版信息
J Clin Microbiol. 2014 Jul;52(7):2625-8. doi: 10.1128/JCM.01025-14. Epub 2014 Apr 23.
Abstract
Among 217 Aeromonas isolates identified by sequencing analysis of their rpoB genes, the accuracy rates of identification of A. dhakensis, A. hydrophila, A. veronii, and A. caviae were 96.7%, 90.0%, 96.7%, and 100.0%, respectively, by the cluster analysis of spectra generated by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
摘要
在通过对217株气单胞菌的rpoB基因进行测序分析鉴定出的菌株中，利用基质辅助激光解吸电离飞行时间质谱法生成的光谱进行聚类分析，嗜水气单胞菌、维氏气单胞菌、豚鼠气单胞菌和达卡气单胞菌的鉴定准确率分别为96.7%、90.0%、96.7%和100.0%。
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