Department of Obstetrics and Gynecology, University of Tennessee Medical Center, Knoxville, TN, USA;
J Thorac Dis. 2013 Jun;5(3):223-7. doi: 10.3978/j.issn.2072-1439.2013.04.14.
A link between pulmonary Ureaplasma spp. colonization in premature infants and bronchopulmonary dysplasia (BPD) exists and could possibly contribute to systemic inflammation.
A prospective cohort study was performed from July 2006 to July 2007 where very low birth weight (VLBW) premature infants were screened at birth. Serum and tracheal aspirate samples were collected during the first 28 days of life that represented the early high-sensitivity C-reactive protein (hs-CRP) group, and follow-up samples obtained between 28-42 days of life were the late hs-CRP group. An Enzyme-linked immunosorbent assay (ELISA) for hs-CRP was performed on serum samples while tracheal aspirates underwent polymerase chain reaction (PCR) analysis for Ureaplasma spp.
A total of 65 patients were screened. 30 patients completed full analysis, 15 died before early and late hs-CRP samples could be obtained, and 20 had incomplete data due to early discharge or transfer. There was no significant difference between all early and late hs-CRP group levels (mg/L), median [interquartile range] 1.019 [0.242, 5.844] vs. 0.773 [0.143, 8.954] (P=0.3958); however, there was a significant difference when comparing Ureaplasma spp. positivity vs. negativity between both groups, median 2.223 [0.398, 7.099] vs. 0.675 [0.219, 4.038] (P=0.0131) for the early group and median 2.335 [0.359, 14.91] vs. 0.2155 [0.122, 2.296] (P=0.03) for the late group, respectively.
VLBW premature infants colonized with Ureaplasma spp. have an elevated hs-CRP, suggestive of a chronic low-grade systemic inflammatory response.
早产儿肺部脲原体定植与支气管肺发育不良（BPD）之间存在关联，并且可能导致全身炎症。
本研究为前瞻性队列研究，于 2006 年 7 月至 2007 年 7 月期间进行，对极低出生体重（VLBW）早产儿进行出生时筛查。在生命的前 28 天内采集血清和气管抽吸样本，代表早期高敏 C 反应蛋白（hs-CRP）组，并在 28-42 天的生命期间采集随访样本，作为晚期 hs-CRP 组。对血清样本进行酶联免疫吸附试验（ELISA）检测 hs-CRP，对气管抽吸物进行脲原体聚合酶链反应（PCR）分析。
共筛查 65 例患者。30 例患者完成了完整分析，15 例患者在获得早期和晚期 hs-CRP 样本之前死亡，20 例患者因早期出院或转移而数据不完整。两组之间的所有早期和晚期 hs-CRP 组水平（mg/L）均无显著差异（P=0.3958）；中位数[四分位距]分别为 1.019[0.242，5.844]与 0.773[0.143，8.954]；然而，当比较两组中脲原体阳性与阴性之间的差异时，早期组的中位数为 2.223[0.398，7.099]与晚期组的 0.675[0.219，4.038]之间存在显著差异（P=0.0131），晚期组的中位数分别为 2.335[0.359，14.91]与 0.2155[0.122，2.296]（P=0.03）。
感染脲原体的 VLBW 早产儿 hs-CRP 升高，提示存在慢性低度全身炎症反应。
