Department of Epidemiology and Public Health, Institute of Infection and Global Health, School of Veterinary Science, Leahurst, University of Liverpool, Liverpool, UK.
28 Kings Road, Crosby, UK.
Epidemiol Infect. 2014 Apr;142(4):861-70. doi: 10.1017/S095026881300160X. Epub 2013 Jul 5.
In a 2-year longitudinal study of adult animals on 15 dairy farms and four sheep farms in Lancashire, UK, Arcobacter spp. were isolated from all farms although not at every sampling occasion. Faecal samples were collected and cultured using standard techniques for isolation of campylobacters. Assignment to species was via PCR assays. Apparent prevalence of Arcobacter spp. was higher in dairy cattle compared to sheep (40.1% vs. 8%, P < 0.001) and in housed cattle compared to cattle at pasture (50.1% vs. 20.9%, P < 0.001). This was reflected in the higher prevalence observed in herds that were housed (n = 4) all year compared to herds that grazed cattle on pasture in the summer and housed cattle in the winter (n = 11) (55.5% vs. 36%, P < 0.001). In the case of sheep, peak prevalence was observed in autumn with increased prevalence also being associated with improving pasture quality. There was an apparent inverse association between the faecal pat prevalence of Arcobacter spp. and Campylobacter jejuni although this may in part be an artefact of laboratory test method sensitivity, whereby a relative increase in the frequency of one bacterial species would reduce the sensitivity of detecting the other.
在英国兰开夏郡的 15 个奶牛场和 4 个绵羊场对成年动物进行的为期 2 年的纵向研究中，虽然并非在每个采样点都能分离到弯曲菌属，但从所有农场中都分离到了弯曲菌属。使用分离弯曲菌的标准技术采集粪便样本并进行培养。通过 PCR 检测来确定菌种。与绵羊相比，奶牛中弯曲菌属的明显流行率更高（40.1%比 8%，P < 0.001），与放牧牛相比，圈养牛中弯曲菌属的流行率更高（50.1%比 20.9%，P < 0.001）。这反映在全年圈养的牛群（n = 4）中的流行率更高，而夏季放牧和冬季圈养的牛群（n = 11）中的流行率较低（55.5%比 36%，P < 0.001）。就绵羊而言，在秋季观察到流行率最高，随着牧场质量的提高，流行率也有所增加。弯曲菌属的粪便外观流行率与空肠弯曲菌之间存在明显的反比关系，尽管这可能部分是实验室检测方法敏感性的假象，因为一种细菌物种的相对增加会降低检测另一种细菌的敏感性。
