Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 13 South Drive, Bethesda, MD 20892, United States.
Curr Opin Neurobiol. 2013 Dec;23(6):1090-7. doi: 10.1016/j.conb.2013.06.008. Epub 2013 Jul 5.
Over the past decade, developmental neuroscience has been transformed by the widespread application of confocal and two-photon fluorescence microscopy. Even greater progress is imminent, as recent innovations in microscopy now enable imaging with increased depth, speed, and spatial resolution; reduced phototoxicity; and in some cases without external fluorescent probes. We discuss these new techniques and emphasize their dramatic impact on neurobiology, including the ability to image neurons at depths exceeding 1mm, to observe neurodevelopment noninvasively throughout embryogenesis, and to visualize neuronal processes or structures that were previously too small or too difficult to target with conventional microscopy.
在过去的十年中，共聚焦和双光子荧光显微镜的广泛应用改变了发展神经科学。随着显微镜技术的最新创新，现在可以实现更深、更快、更高空间分辨率的成像，减少光毒性，并且在某些情况下无需外部荧光探针，这将使进展更加迅速。我们讨论了这些新技术，并强调了它们对神经生物学的巨大影响，包括在超过 1mm 的深度成像神经元，在整个胚胎发育过程中无创观察神经发育，以及可视化以前由于太小或难以用传统显微镜定位而无法观察到的神经元过程或结构的能力。
