Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
Dev Cell. 2012 Dec 11;23(6):1103-10. doi: 10.1016/j.devcel.2012.10.003.
The recent invention of superresolution microscopy has brought up much excitement in the biological research community. Here, we focus on stochastic optical reconstruction microscopy/photoactivated localization microscopy (STORM/PALM) to discuss the challenges in applying superresolution microscopy to the study of developmental biology, including tissue imaging, sample preparation artifacts, and image interpretation. We also summarize new opportunities that superresolution microscopy could bring to the field of developmental biology.
最近超分辨率显微镜的发明在生物研究界引起了极大的兴奋。在这里，我们专注于随机光学重建显微镜/光激活定位显微镜（STORM/PALM）来讨论将超分辨率显微镜应用于发育生物学研究中的挑战，包括组织成像、样品制备伪影和图像解释。我们还总结了超分辨率显微镜可能为发育生物学领域带来的新机遇。
