Zhu Hongbin, Liu Shu, Li Xue, Song Fengrui, Liu Zhiqiang, Liu Shuying
Chemical Biology Laboratory & Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
Anal Bioanal Chem. 2013 Sep;405(23):7437-45. doi: 10.1007/s00216-013-7153-1. Epub 2013 Jul 7.
A novel fingerprinting method, bioactivity fingerprint analysis, based on an ultrafiltration-ultraperformance liquid chromatography-multistage tandem mass spectrometry (UPLC-MS(n)) method is proposed for the quality control of herbal medicines from the bioactivity viewpoint concerning the efficacy of herbal medicines. The bioactivity fingerprints reflecting the anti-inflammatory activities of radix Aconiti and radix Aconiti preparata were established. With use of ultrafiltration UPLC-MS(n), 11 cyclooxygenase-2 ligands from radix Aconiti preparata and 14 cyclooxygenase-2 ligands from radix Aconiti were found after incubation with cyclooxygenase-2. Twelve of the cyclooxygenase-2 ligands were identified by the ultraperformance UPLC-MS(n) method. The enrichment factor of each peak in the bioactivity fingerprint was calculated and was demonstrated to be characteristic, which makes bioactivity fingerprint analysis for the quality control of herbal medicines possible from the viewpoint of their bioactivities.
提出了一种基于超滤-超高效液相色谱-多级串联质谱（UPLC-MS(n)）方法的新型指纹图谱方法——生物活性指纹图谱分析，从中药功效的生物活性角度对中药进行质量控制。建立了反映附子和制附子抗炎活性的生物活性指纹图谱。通过超滤UPLC-MS(n)，在与环氧化酶-2孵育后，从制附子中发现了11种环氧化酶-2配体，从附子中发现了14种环氧化酶-2配体。其中12种环氧化酶-2配体通过超高效UPLC-MS(n)方法得到鉴定。计算了生物活性指纹图谱中各峰的富集因子，并证明其具有特征性，这使得从生物活性角度对中药进行质量控制的生物活性指纹图谱分析成为可能。
