1. Advanced Personalized Diagnostics LLC, 6006 Bangor Drive, Alexandria, VA 22303, USA. ; 2. Angiogenesis Core Facility, Advanced Technology Center, Room 115, Center for Cancer Research, National Cancer Institute, 8717 Grovemont Circle, Bethesda, MD 20892-4605, USA.
J Cancer. 2013 Jun 26;4(5):402-15. doi: 10.7150/jca.6780. Print 2013.
We have developed novel phenotypic fluorescent three-dimensional co-culture platforms that efficiently and economically screen anti-angiogenic/anti-metastatic drugs on a high-throughput scale. Individual cell populations can be identified and isolated for protein/gene expression profiling studies and cellular movement/interactions can be tracked by time-lapse cinematography. More importantly, these platforms closely parallel the in vivo angiogenic and metastatic outcomes of a given tumor xenograft in the nude mouse model but, unlike in vivo models, our co-culture platforms produce comparable results in five to nine days. Potentially, by incorporating cancer patient biopsies, the co-culture platforms should greatly improve the effectiveness and efficiency of personalized chemotherapy.
我们开发了新颖的表型荧光三维共培养平台，可在高通量水平上高效且经济地筛选抗血管生成/抗转移药物。可以对单个细胞群体进行鉴定和分离，以进行蛋白质/基因表达谱研究，并且可以通过延时摄影术跟踪细胞运动/相互作用。更重要的是，这些平台与裸鼠模型中特定肿瘤异种移植物的体内血管生成和转移结果非常相似，但与体内模型不同的是，我们的共培养平台在五到九天内产生可比的结果。通过合并癌症患者活检，共培养平台有望大大提高个性化化疗的效果和效率。
