冷冻保存样本的非侵入性质量控制
Noninvasive Quality Control of Cryopreserved Samples.
作者信息
Dörr Daniel, Stracke Frank, Zimmermann Heiko
出版信息
Biopreserv Biobank. 2012 Dec;10(6):529-31. doi: 10.1089/bio.2012.0011.
Abstract
We present a novel noninvasive technology for quality control in biobanking. We implemented a contactless optical in situ method with a remote detection unit. The method detects physical and chemical changes by emission spectroscopy. In the present study, ice formation in a vitrified sample is revealed by Raman scattering. The technology allows us to monitor sample quality during cold storage and to assess the sample state after preservation, storage, or transport without the need for thawing.
摘要
我们提出了一种用于生物样本库质量控制的新型非侵入性技术。我们采用了一种带有远程检测单元的非接触式光学原位方法。该方法通过发射光谱检测物理和化学变化。在本研究中，通过拉曼散射揭示了玻璃化样本中的结冰情况。这项技术使我们能够在冷藏期间监测样本质量，并在保存、储存或运输后无需解冻即可评估样本状态。
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