Heart Research Institute, Newtown, NSW 2042, Australia ; Faculty of Pharmacy, University of Sydney, Camperdown, NSW 2006, Australia ; Department of Endocrinology, Dezhou People's Hospital, Dezhou, Shandong 253014, China.
Evid Based Complement Alternat Med. 2013;2013:146142. doi: 10.1155/2013/146142. Epub 2013 Jun 16.
Introduction. Hepatic inflammation underlies the pathogenesis of chronic diseases such as insulin resistance and type 2 diabetes mellitus. S-[6]-Gingerol has been shown to have anti-inflammatory properties. Important inflammatory mediators of interleukins include nuclear factor κ B (NF κ B) and cyclooxygenase 2 (COX2). We now explore the mechanism of anti-inflammatory effects of S-[6]-gingerol in liver cells. Methods. HuH7 cells were stimulated with IL1β to establish an in vitro hepatic inflammatory model. Results. S-[6]-Gingerol attenuated IL1β-induced inflammation and oxidative stress in HuH7 cells, as evidenced by decreasing mRNA levels of inflammatory factor IL6, IL8, and SAA1, suppression of ROS generation, and increasing mRNA levels of DHCR24. In addition, S-[6]-gingerol reduced IL1β-induced COX2 upregulation as well as NF κ B activity. Similar to the protective effects of S-[6]-gingerol, both NS-398 (a selective COX2 inhibitor) and PDTC (a selective NF κ B inhibitor) suppressed mRNA levels of IL6, IL8, and SAA1. Importantly, PDTC attenuated IL1β-induced overexpression of COX2. Of particular note, the protective effect of S-[6]-gingerol against the IL1β-induced inflammatory response was similar to that of BHT, an ROS scavenger. Conclusions. The findings of this study demonstrate that S-[6]-gingerol protects HuH7 cells against IL1β-induced inflammatory insults through inhibition of the ROS/NF κ B/COX2 pathway.
简介。肝脏炎症是胰岛素抵抗和 2 型糖尿病等慢性疾病发病机制的基础。S-[6]-姜烯酚具有抗炎特性。白细胞介素的重要炎症介质包括核因子 κ B（NF κ B）和环氧化酶 2（COX2）。我们现在探讨 S-[6]-姜烯酚在肝细胞中抗炎作用的机制。方法。用 IL1β 刺激 HuH7 细胞建立体外肝炎症模型。结果。S-[6]-姜烯酚减轻了 HuH7 细胞中 IL1β 诱导的炎症和氧化应激，表现在炎症因子 IL6、IL8 和 SAA1 的 mRNA 水平降低，ROS 生成减少，DHCR24 的 mRNA 水平增加。此外，S-[6]-姜烯酚降低了 IL1β 诱导的 COX2 上调以及 NF κ B 活性。与 S-[6]-姜烯酚的保护作用相似，NS-398（一种选择性 COX2 抑制剂）和 PDTC（一种选择性 NF κ B 抑制剂）均抑制了 IL6、IL8 和 SAA1 的 mRNA 水平。重要的是，PDTC 减弱了 IL1β 诱导的 COX2 过表达。值得注意的是，S-[6]-姜烯酚对 IL1β 诱导的炎症反应的保护作用与 ROS 清除剂 BHT 相似。结论。本研究结果表明，S-[6]-姜烯酚通过抑制 ROS/NF κ B/COX2 途径保护 HuH7 细胞免受 IL1β 诱导的炎症损伤。
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