从山楂种子中分离细胞毒性化合物。
Isolation of cytotoxic compounds from the seeds of Crataegus pinnatifida.
机构信息
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Chin J Nat Med. 2013 Jul;11(4):411-4. doi: 10.1016/S1875-5364(13)60061-8.
AIM
To study the chemical constituents and bioactivity of the seeds of Crataegus pinnatifida.
METHODS
The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography, and preparative HPLC. Their structures were elucidated on the basis of spectroscopic methods. In addition, the cytotoxic activities of compounds 1-4 were investigated on OPM2 and RPMI-8226 cells.
RESULTS
Four compounds were obtained and their structures were identified as (7S, 8S)-4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(hydroxymethyl)ethoxy]-3, 5-dimethoxybenzaldehyde (1), (+)-balanophonin (2), erythro-guaiacylglycerol-β-coniferyl aldehyde ether (3), buddlenol A (4).
CONCLUSION
Compound 1 is a novel norlignan, while compounds 1-4 exhibited marginal inhibition on the proliferation of OPM2 and RPMI-8226 cells.
目的
研究山楂种子的化学成分和生物活性。
方法
采用大孔吸附树脂 D101、硅胶和 ODS 柱色谱、制备高效液相色谱等方法对化学成分进行分离纯化。根据光谱数据鉴定其结构。此外，还研究了化合物 1-4 对 OPM2 和 RPMI-8226 细胞的细胞毒性。
结果
分离得到 4 个化合物，鉴定它们的结构分别为（7S，8S）-4-[2-羟基-2-（4-羟基-3-甲氧基苯基）-1-（羟甲基）乙氧基]-3,5-二甲氧基苯甲醛（1）、（+）-balanophonin（2）、erythro-愈创木基甘油-β-松柏醛醚（3）、buddlenol A（4）。
结论
化合物 1 为新的愈创木烷型木脂素，化合物 1-4 对 OPM2 和 RPMI-8226 细胞的增殖有轻微抑制作用。
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