College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
Chin J Nat Med. 2013 May;11(3):269-73. doi: 10.1016/S1875-5364(13)60027-8.
To investigate the chemical constituents from the leaves of Broussonetia papyrifera.
The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography and preparative HPLC. Their structures were elucidated on the basis of 1D and 2D NMR analyses. In addition, their cytotoxic activity against human hepatoma carcinoma cells (HepG-2) were evaluated by the MTT method. Furthermore, RP-HPLC and colorimetric methods were used for the analysis of cosmosiin and total flavonoids.
A new lignan, together with five known compounds were obtained, and their structures were characterized as (+)-pinoresinol-4'-O-β-D-glucopyranosyl-4″-O-β-D-apiofuranoside (1), cosmosiin (2), luteolin-7-O-β-D-glucopyranoside (3), liriodendrin (4), 3, 5, 4'-trihydroxy-bibenzyl-3-O-β-D-glucoside (5), and apigenin-6-C-β-D-glucopyranside (6). Furthermore, RP-HPLC and colorimetric methods were established for the analysis of cosmosiin and total flavonoids.
Compound 1 was a new lignan, and compounds 5 and 6 were isolated for the first time from the title plant. Compounds 1, 4 and 6 showed definite activities against HepG-2, while the other compounds didn't show inhibitory effects. The optimal harvest time of B. papyrifera (L.) Vent. is September.
研究构树叶的化学成分。
采用大孔吸附树脂 D101、硅胶和 ODS 柱色谱及制备 HPLC 等方法对化学成分进行分离纯化，根据 1D 和 2D NMR 分析鉴定其结构。此外，采用 MTT 法评价其对人肝癌细胞（HepG-2）的细胞毒性。还采用反相高效液相色谱法和比色法分析了星宿菜苷和总黄酮的含量。
从构树叶中分离得到一个新的木脂素，以及 5 个已知化合物，其结构分别鉴定为（+）-松脂醇-4'-O-β-D-吡喃葡萄糖基-4″-O-β-D-吡喃芹菜糖基（1）、星宿菜苷（2）、木樨草素-7-O-β-D-吡喃葡萄糖苷（3）、紫铆因（4）、3,5,4'-三羟基联苯-3-O-β-D-葡萄糖苷（5）和芹菜素-6-C-β-D-吡喃葡萄糖苷（6）。此外，还建立了反相高效液相色谱法和比色法分析星宿菜苷和总黄酮的含量。
化合物 1 为新木脂素，化合物 5 和 6 为该植物首次分离得到。化合物 1、4 和 6 对 HepG-2 具有一定的活性，而其他化合物则没有抑制作用。构树（L.）Vent. 的最佳收获时间为 9 月。
