The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Biochem Biophys Res Commun. 2013 Aug 9;437(4):544-9. doi: 10.1016/j.bbrc.2013.06.112. Epub 2013 Jul 8.
Glyphosate is a broad spectrum, non-selective herbicide which has been widely used for weed control. Much work has focused on elucidating the high accumulation of glyphosate in shoot apical bud (shoot apex). However, to date little is known about the molecular mechanisms of the sensitivity of shoot apical bud to glyphosate. Global gene expression profiling of the soybean apical bud response to glyphosate treatment was performed in this study. The results revealed that the glyphosate inhibited tryptophan biosynthesis of the shikimic acid pathway in the soybean apical bud, which was the target site of glyphosate. Glyphosate inhibited the expression of most of the target herbicide site genes. The promoter sequence analysis of key target genes revealed that light responsive elements were important regulators in glyphosate induction. These results will facilitate further studies of cloning genes and molecular mechanisms of glyphosate on soybean shoot apical bud.
草甘膦是一种广谱、非选择性除草剂，已被广泛用于杂草控制。大量工作集中在阐明草甘膦在茎尖芽（茎尖）中的高积累。然而，迄今为止，对于茎尖芽对草甘膦敏感性的分子机制知之甚少。本研究对大豆茎尖芽对草甘膦处理的反应进行了全基因表达谱分析。结果表明，草甘膦抑制了大豆茎尖芽中莽草酸途径中天冬氨酸的生物合成，这是草甘膦的靶标位点。草甘膦抑制了大多数靶标除草剂位点基因的表达。关键靶标基因启动子序列分析表明，光响应元件是草甘膦诱导的重要调节剂。这些结果将有助于进一步研究大豆茎尖芽上草甘膦基因的克隆和分子机制。
