Yoshino K, Kishie T, Hashimoto M, Yanagi K
Arch Virol. 1975;47(1):31-8. doi: 10.1007/BF01315590.
HEP Flury strain of rabies virus was propagated in chick embryo cells under maintenance media of different pH. It was found that viral growth was better and reached a markedly higher maximum titer when the initial pH of maintenance medium was 8.2 to 9.0 than when it was 7.4. The enhancement of viral growth was not ascribable to mere neutralization of acids produced from infected cells, because the different media became almost equally neutral within an early phase of growth curve. Serial passage of the virus in chick embryo cells using pH 8.2 maintenance medium resulted in altered growth characteristics of the progeny virus; first, the virus so passaged could now grow equally well under alkaline and neutral maintenance media, and, secondly, autointerference observable with the parent virus eventually lowered virus yield when neutral maintenance medium was used, but this effect of undiluted passage was eliminated by the use of pH 8.2 maintenance medium.
狂犬病病毒HEP弗勒里毒株在不同pH值的维持培养基中于鸡胚细胞内增殖。结果发现，当维持培养基的初始pH值为8.2至9.0时，病毒生长更好且达到的最高滴度明显高于pH值为7.4时。病毒生长的增强并非仅仅归因于对感染细胞产生的酸的中和作用，因为在生长曲线的早期阶段，不同的培养基几乎变得同样呈中性。使用pH值为8.2的维持培养基在鸡胚细胞中对病毒进行连续传代，导致子代病毒的生长特性发生改变；首先，如此传代后的病毒现在在碱性和中性维持培养基下生长得同样良好，其次，亲本病毒可观察到的自身干扰在使用中性维持培养基时最终会降低病毒产量，但通过使用pH值为8.2的维持培养基，这种未稀释传代的影响得以消除。
