TRPC1 和 TRPC3 在骨骼肌萎缩和再生长过程中的适应性反应。

Adaptive responses of TRPC1 and TRPC3 during skeletal muscle atrophy and regrowth.

机构信息

Muscle Physiology Laboratory, Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

出版信息

Muscle Nerve. 2014 May;49(5):691-9. doi: 10.1002/mus.23952.

DOI:10.1002/mus.23952

PMID:23852583

Abstract

INTRODUCTION

We assessed the time-dependent changes of transient receptor potential canonical type 1 (TRPC1) and TRPC3 expression and localization associated with muscle atrophy and regrowth in vivo.

METHODS

Mice were subjected to hindlimb unloading for 7 or 14 days (7U, 14U) followed by 3, 7, or 14 days of reloading (3R, 7R, 14R).

RESULTS

Soleus muscle mass and tetanic force were reduced significantly at 7U and 14U and recovered by 14R. Recovery of muscle fiber cross-sectional area was observed by 28R. TRPC1 mRNA was unaltered during the unloading-reloading period. However, protein expression remained depressed through 14R. Decreased localization of TRPC1 to the sarcolemma was observed. TRPC3 mRNA and protein expression levels were decreased significantly during the early phase of reloading.

CONCLUSIONS

Given the known role of these channels in muscle development, changes observed in TRPC1 and TRPC3 may relate closely to muscle atrophy and remodeling processes.

摘要

简介

本研究旨在评估瞬时受体电位经典型 1（TRPC1）和 TRPC3 表达和定位在体内肌肉萎缩和再生长过程中的时程变化。

方法

通过后肢去负荷处理建立小鼠模型，分别处理 7 天（7U）和 14 天（14U），然后再进行 3 天（3R）、7 天（7R）和 14 天（14R）的负荷恢复。

结果

7U 和 14U 时比目鱼肌质量和强直张力显著降低，14R 时恢复。28R 时观察到肌纤维横截面积的恢复。在去负荷-再负荷期间，TRPC1mRNA 没有改变。然而，蛋白质表达一直持续到 14R 时仍受到抑制。观察到 TRPC1 向肌膜的定位减少。在再负荷的早期阶段，TRPC3mRNA 和蛋白表达水平显著降低。