Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, Seoul 06591, Korea.
Cells. 2020 Apr 1;9(4):850. doi: 10.3390/cells9040850.
Ca itself or Ca-dependent signaling pathways play fundamental roles in various cellular processes from cell growth to death. The most representative example can be found in skeletal muscle cells where a well-timed and adequate supply of Ca is required for coordinated Ca-dependent skeletal muscle functions, such as the interactions of contractile proteins during contraction. Intracellular Ca movements between the cytosol and sarcoplasmic reticulum (SR) are strictly regulated to maintain the appropriate Ca supply in skeletal muscle cells. Added to intracellular Ca movements, the contribution of extracellular Ca entry to skeletal muscle functions and its significance have been continuously studied since the early 1990s. Here, studies on the roles of channel proteins that mediate extracellular Ca entry into skeletal muscle cells using skeletal myoblasts, myotubes, fibers, tissue, or skeletal muscle-originated cell lines are reviewed with special attention to the proposed functions of transient receptor potential canonical proteins (TRPCs) as store-operated Ca entry (SOCE) channels under normal conditions and the potential abnormal properties of TRPCs in muscle diseases such as Duchenne muscular dystrophy (DMD).
钙本身或钙依赖的信号通路在细胞生长到死亡的各种细胞过程中发挥着基本作用。最具代表性的例子可以在骨骼肌细胞中找到，在骨骼肌细胞中，钙的适时和充足供应对于协调的钙依赖的骨骼肌功能是必需的，例如在收缩期间收缩蛋白的相互作用。细胞溶质和肌浆网（SR）之间的细胞内钙运动受到严格调节，以维持骨骼肌细胞中的适当钙供应。除了细胞内钙运动之外，自 20 世纪 90 年代初以来，人们一直在不断研究细胞外钙进入对骨骼肌功能的贡献及其意义。在这里，使用成肌细胞、肌管、纤维、组织或源自骨骼肌的细胞系，综述了介导细胞外钙进入骨骼肌细胞的通道蛋白的作用，特别关注瞬时受体电位经典蛋白（TRPCs）在正常条件下作为储存操作钙进入（SOCE）通道的作用，以及在肌肉疾病（如杜氏肌营养不良症（DMD））中 TRPC 的潜在异常特性。
