National Engineering Laboratory for Livestock and Poultry Breeding, Plateau Animal Genetic Resources Center, China Agriculture University, Beijing 100193, China.
College of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China.
Cells. 2021 Jan 13;10(1):147. doi: 10.3390/cells10010147.
In animals, muscle growth is a quantitative trait controlled by multiple genes. Previously, we showed that the transient receptor potential channel 1 () gene was differentially expressed in muscle tissues between pig breeds with divergent growth traits base on RNA-seq. Here, we characterized expression profiles in different tissues and pig breeds and showed that was highly expressed in the muscle. We found two single nucleotide polymorphisms (SNPs) (C-1763T and C-1604T) in that could affect the promoter region activity and regulate pig growth rate. Functionally, we used RNAi and overexpression to illustrate that promotes myoblast proliferation, migration, differentiation, fusion, and muscle hypertrophy while inhibiting muscle degradation. These processes may be mediated by the activation of Wnt signaling pathways. Altogether, our results revealed that might promote muscle growth and development and plays a key role in Wnt-mediated myogenesis.
在动物中，肌肉生长是由多个基因控制的数量性状。此前，我们通过 RNA-seq 研究表明，在生长性状不同的猪品种之间，瞬时受体电位通道 1（）基因在肌肉组织中差异表达。在这里，我们描述了不同组织和猪品种中的表达谱，结果表明在肌肉中高度表达。我们在中发现了两个可能影响启动子区域活性并调节猪生长速度的单核苷酸多态性（SNP）（C-1763T 和 C-1604T）。功能上，我们使用 RNAi 和过表达来阐明促进成肌细胞增殖、迁移、分化、融合和肌肉肥大，同时抑制肌肉降解。这些过程可能是通过激活 Wnt 信号通路来介导的。总的来说，我们的结果表明可能促进肌肉生长和发育，并在 Wnt 介导的成肌发生中发挥关键作用。
