Kadkhodazadeh Mahdi, Ebadian Ahmad Reza, Amid Reza, Youssefi Navid, Mehdizadeh Amir Reza
Department of Periodontics, Dental Research Center, Dental School, Shahid Beheshti Medical University, Tehran, Iran.
Acta Med Iran. 2013 Jul 13;51(6):353-8.
Gene polymorphism of cytokines influencing their function has been known as a contributing factor in the pathogenesis of inflammatory diseases of the tooth and implant supporting tissues. The aim of this study was to investigate the association of IL-17R gene polymorphism (rs879576) with chronic periodontitis and periimplantitis in an Iranian population. 73 patients with chronic periodontitis, 37 patients with periimplantitis and 83 periodontally healthy patients were enrolled in this study. 5cc blood was obtained from each subject's arm vein and transferred to tubes containing EDTA. Genomic DNA was extracted using Miller's Salting Out technique. The DNA was transferred into 96 division plates, transported to Kbioscience Institute in United Kingdom and analyzed using the Kbioscience Competitive Allele Specific PCR (KASP) technique. Chi-square and Kruskal Wallis tests were used to analyze differences in the expression of genotypes and frequency of alleles in disease and control groups (P-Value less than 0.05 was considered statistically significant). There were no significant differences between periodontitis, periimplantitis with AA, GG, GA genotype of IL-17R gene (P=0.8239). Also comparison of frequency of alleles in SNP rs879576 of IL-17R gene between the chronic periodontitis group and periimplantitis group did not revealed statistically significant differences (P=0.8239). The enigma of IL-17 and its polymorphism-role in periodontitis and periimplantitis is yet to be investigated more carefully throughout further research but this article demonstrates that polymorphism of IL-17R plays no significant role in incidence of chronic periodontitis and Periimplantitis.
影响细胞因子功能的基因多态性被认为是牙齿和种植体支持组织炎症性疾病发病机制中的一个促成因素。本研究的目的是调查伊朗人群中白细胞介素-17受体（IL-17R）基因多态性（rs879576）与慢性牙周炎和种植体周围炎的关联。本研究纳入了73例慢性牙周炎患者、37例种植体周围炎患者和83例牙周健康患者。从每位受试者的手臂静脉采集5毫升血液，并转移至含有乙二胺四乙酸（EDTA）的试管中。使用米勒盐析技术提取基因组DNA。将DNA转移到96孔板中，运至英国的Kbioscience研究所，并使用Kbioscience竞争性等位基因特异性PCR（KASP）技术进行分析。采用卡方检验和克鲁斯卡尔-沃利斯检验分析疾病组和对照组中基因型表达和等位基因频率的差异（P值小于0.05被认为具有统计学意义）。IL-17R基因的AA、GG、GA基因型在牙周炎组和种植体周围炎组之间没有显著差异（P=0.8239）。此外，慢性牙周炎组和种植体周围炎组之间IL-17R基因SNP rs879576等位基因频率的比较也未显示出统计学上的显著差异（P=0.8239）。白细胞介素-17及其多态性在牙周炎和种植体周围炎中的作用之谜还有待通过进一步研究更仔细地探究，但本文表明IL-17R多态性在慢性牙周炎和种植体周围炎的发病中不起显著作用。
