Department of Electrical Engineering, Polystim Neurotechnologies Laboratory, Ecole Polytechnique de Montreal, Montreal, QC H3T 1J4 Canada.
IEEE Trans Biomed Circuits Syst. 2012 Apr;6(2):120-32. doi: 10.1109/TBCAS.2012.2185844.
We present in this paper a new Lab-on-Chip (LoC) architecture for dielectrophoresis-based cell manipulation, detection, and capacitive measurement. The proposed LoC is built around a CMOS full-custom chip and a microfluidic structure. The CMOS chip is used to deliver all parameters required to control the dielectrophoresis (DEP) features such as frequency, phase, and amplitude of signals spread on in-channel electrodes of the LoC. It is integrated to the LoC and experimental results are related to micro and nano particles manipulation and detection in a microfluidic platform. The proposed microsystem includes an on-chip 27-bit frequency divider, a digital phase controller with a 3.6° phase shift resolution and a 2.5 V dynamic range. The sensing module is composed of a 3 × 3 capacitive sensor array with 10 fF per mV sensitivity, and a dynamic range of 1.5 V. The obtained results show an efficient nano and micro-particles (PC05N, PA04N and PS03N) separation based on frequency segregation with low voltages less than 1.7 V and a fully integrated and reconfigurable system.
本文提出了一种新的基于介电泳的芯片实验室（Lab-on-Chip，LoC）架构，用于细胞操作、检测和电容测量。所提出的 LoC 基于 CMOS 全定制芯片和微流控结构构建。CMOS 芯片用于提供控制介电泳（Dielectrophoresis，DEP）的所有参数，例如信号在 LoC 通道电极上的频率、相位和幅度。它与 LoC 集成，实验结果与微纳粒子在微流控平台上的操作和检测有关。所提出的微系统包括片上 27 位分频器、具有 3.6°相移分辨率和 2.5 V 动态范围的数字相控制器。传感模块由 3×3 电容传感器阵列组成，灵敏度为 10 fF/mV，动态范围为 1.5 V。所获得的结果表明，基于频率分离的高效纳米和微粒子（PC05N、PA04N 和 PS03N）分离，电压低于 1.7 V，以及完全集成和可重构的系统。
