Pintér Réka, Madai Mónika, Vadkerti Edit, Németh Viktória, Oldal Miklós, Kemenesi Gábor, Dallos Bianka, Gyuranecz Miklós, Kiss Gábor, Bányai Krisztián, Jakab Ferenc
Virological Research Group, János Szentágothai Research Center, University of Pécs, Pécs, Hungary.
Ticks Tick Borne Dis. 2013 Sep;4(5):427-31. doi: 10.1016/j.ttbdis.2013.04.008. Epub 2013 Jul 13.
Tick-borne encephalitis virus (TBEV) is an arthropod-borne viral pathogen causing infections in Europe and is responsible for most arbovirus central nervous system infections in Hungary. Assessing the TBEV prevalence in ticks through detection of genomic RNA is a broadly accepted approach to estimate the transmission risk from a tick bite. For this purpose, 2731 ticks were collected from the neighboring area of the town of Dévaványa, located in southeastern Hungary, which is considered a low-risk-transmission area for TBEV. Altogether, 2300 ticks were collected from the vegetation, while 431 were collected from rodents. Samples were pooled and then screened for TBEV with a newly designed semi-nested RT-PCR (RT-snPCR) targeting the NS1 genomic region. PCR results were confirmed by direct sequencing of the second round amplicons. Among the 3 different collected tick species (Ixodes ricinus, Haemaphysalis concinna, Dermacentor marginatus), I. ricinus was the only species that tested positive for TBEV. TBEV-positive ticks were collected from small mammals or from the vegetation. One nymphal pool and 4 larval pools tested positive for TBEV. The only positive nymphal pool was unfed and came from vegetation, while ticks of the 4 positive larval pools were collected from rodents. Minimal TBEV prevalence in ticks was 0.08% for unfed nymphs and 0.78% for feeding larvae. Our results indicate that further long-term investigations on the occurrence of TBEV are needed to better describe the geographic distribution and the prevalence of infected ticks in Hungary.
蜱传脑炎病毒（TBEV）是一种节肢动物传播的病毒病原体，在欧洲引发感染，且是匈牙利大多数虫媒病毒中枢神经系统感染的病因。通过检测基因组RNA来评估蜱中TBEV的流行情况是一种广泛认可的估计蜱叮咬传播风险的方法。为此，从匈牙利东南部德瓦瓦尼亚镇周边地区收集了2731只蜱，该地区被认为是TBEV低风险传播地区。总共从植被中收集了2300只蜱，从啮齿动物身上收集了431只。样本合并后，用新设计的靶向NS1基因组区域的半巢式逆转录聚合酶链反应（RT-snPCR）筛选TBEV。通过第二轮扩增子的直接测序确认PCR结果。在3种不同采集的蜱种（蓖麻硬蜱、嗜群血蜱、边缘革蜱）中，蓖麻硬蜱是唯一检测出TBEV呈阳性的物种。TBEV阳性蜱从小型哺乳动物或植被中采集到。一个若虫样本池和4个幼虫样本池检测出TBEV呈阳性。唯一呈阳性的若虫样本池未进食，来自植被，而4个阳性幼虫样本池的蜱是从啮齿动物身上采集的。未进食若虫的蜱中TBEV最低流行率为0.08%，进食幼虫的为0.78%。我们的结果表明，需要对TBEV的发生情况进行进一步的长期调查，以更好地描述匈牙利受感染蜱的地理分布和流行情况。
