Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, Columbus, OH 43215, USA.
J Biomech. 2013 Sep 3;46(13):2277-82. doi: 10.1016/j.jbiomech.2013.06.013. Epub 2013 Jul 13.
Multiple murine models have proven useful in studying the natural history of neovessel development in the tissue engineering of vascular grafts. Nevertheless, to better understand longitudinal changes in the biomechanics of such neovessels, we must first quantify native tissue structure and properties. In this paper, we present the first biaxial mechanical data for, and nonlinear constitutive modeling of, &QJ;the inferior vena cava from two models used in tissue engineering: wild-type C57BL/6 and immunodeficient CB-17 SCID/bg mice. Results show that inferior vena cava from the latter are significantly stiffer in the circumferential direction, both materially (as assessed by a stored energy function) and structurally (as assessed by the compliance), despite a lower intramural content of fibrillar collagen and similar wall thickness. Quantifying the natural history of neovessel development in different hosts could lead to increased insight into the mechanisms by which cells fashion and maintain extracellular matrix in order to match best the host stiffness while ensuring sufficient vascular integrity.
多种鼠类模型已被证明在研究血管移植物组织工程中新生血管的自然史方面非常有用。然而，为了更好地了解此类新生血管的生物力学的纵向变化，我们必须首先量化天然组织的结构和特性。在本文中，我们提出了第一个用于组织工程中两种模型的下腔静脉的双轴力学数据和非线性本构模型：野生型 C57BL/6 和免疫缺陷 CB-17 SCID/bg 小鼠。结果表明，后者的下腔静脉在周向方向上明显更硬，无论是在材料方面（通过储能函数评估）还是在结构方面（通过顺应性评估），尽管纤维胶原的壁内含量较低，壁厚度相似。量化不同宿主中新生血管发育的自然史，可以更深入地了解细胞塑造和维持细胞外基质的机制，以便在确保足够的血管完整性的同时，最好地匹配宿主的刚度。
