Marakusha B I, Petrovskaia V G
Zh Mikrobiol Epidemiol Immunobiol. 1990 Apr(4):11-7.
Neamine-resistant mutants were obtained from S. abortus ovis virulent strain. These mutants were divided into three classes according to their sensitivity to streptomycin: mutants completely retaining their sensitivity, mutants sensitive to moderate and high doses of the antibiotic. On the basis of genetic analysis carried out with the use of bacteriophage P22, the Near mutation of class Near 100 Strr 500 mutants was identified as nea B, and the Near mutation of class Near 100 Strs, as nea A. The study showed a decreased virulence of Salmonella transductants that acquired both neamine-resistant mutation of the two classes and streptomycin-resistant mutation. The streptomycin-resistant mutation produced no changes in the virulence of these bacteria. According to the results of experiments on mice, mutants of the two classes under study were found to possess protective activity.
从羊流产沙门氏菌强毒株中获得了新霉素抗性突变体。根据这些突变体对链霉素的敏感性，将它们分为三类：完全保留其敏感性的突变体、对中等剂量和高剂量抗生素敏感的突变体。在使用噬菌体P22进行遗传分析的基础上，100 Strr 500类突变体的Near突变被鉴定为nea B，而100 Strs类的Near突变被鉴定为nea A。研究表明，获得两类新霉素抗性突变和链霉素抗性突变的沙门氏菌转导子的毒力降低。链霉素抗性突变并未使这些细菌的毒力发生变化。根据对小鼠的实验结果，发现所研究的两类突变体具有保护活性。
