Vienna University of Technology, Dept. of Bioelectronics, FKE, Floragasse 7, 1040 Vienna, Austria; Center for Brain Research, Medical University of Vienna, Spitalgasse 4, 1090 Vienna, Austria.
J Biophotonics. 2014 Jan;7(1-2):117-25. doi: 10.1002/jbio.201300048. Epub 2013 Jul 17.
Using a combination of achromatic aspheric optical elements and achromatic cylindrical lenses, we developed an improved laser light sheet generator for two-side illumination ultramicroscopy. This light sheet generator has a much longer Rayleigh range, a more uniform spatial intensity distribution along x -, y - and z-axis, and reduced aberrations than the standard system consisting of a slit aperture and a single cylindrical lens, which is commonly used in light sheet microscopy. As there is no truncation of the beam by a slit aperture in our design the laser energy is used more efficiently. Applying this light sheet generator to ultramicroscopy of chemically cleared biological samples, such as Drosophila, dissected mouse hippocampi, and entire mouse brains, we achieved a markedly improved resolution of fine details.
我们使用消色差非球面光学元件和消色差柱面透镜的组合，开发了一种改进的用于双面照明超微显微镜的激光光片发生器。与通常用于光片显微镜的由狭缝孔径和单个柱面透镜组成的标准系统相比，这种光片发生器具有更长的瑞利范围、更均匀的空间强度分布沿 x 、 y 和 z 轴，并且像差更小。由于在我们的设计中没有狭缝孔径对光束的截断，因此激光能量的利用效率更高。将这种光片发生器应用于化学清除的生物样本的超微显微镜观察，如果蝇、解剖的小鼠海马体和整个小鼠大脑，我们实现了精细细节的分辨率的显著提高。
