泰国普罗威登斯菌新种，从海产品加工废水中分离得到。
Providencia thailandensis sp. nov., isolated from seafood processing wastewater.
作者信息
Khunthongpan Suwannee, Sumpavapol Punnanee, Tanasupawat Somboon, Benjakul Soottawat, H-Kittikun Aran
机构信息
Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Thailand.
出版信息
J Gen Appl Microbiol. 2013;59(3):185-90. doi: 10.2323/jgam.59.185.
The bacterial strain C1112(T) was isolated from seafood processing wastewater collected from a treatment pond of the seafood factory in Songkhla Province, Thailand. Phylogenetic analysis based on concatenated sequences from the 16S rRNA gene and five housekeeping genes, fusA, lepA, leuS, gyrB and ileS respectively showed that the strain C1112(T) belonged to the genus Providencia, and share 91.75% similarity with P. stuartii DSM 4539(T). DNA-DNA hybridization between the strain C1112(T) and P. stuartii KCTC 2568(T) was 48.1% relatedness. Moreover, some results from biochemical properties indicated that the strain C1112(T) was distinguished from the phylogenetically closest relatives. The major fatty acids of the strain C1112(T) were C16:0, iso-C15:0, C14:0 and C17:0 cyclo and the DNA G+C content was 41 mol%. Based on the genotypic and phenotypic considerations, it should be classified as a novel species of the genus Providencia for which the name Providencia thailandensis sp. nov. is proposed. The type strain is C1112(T) (= KCTC 23281(T) =NBRC 106720(T)).
菌株C1112(T)是从泰国宋卡府一家海鲜加工厂处理池收集的海鲜加工废水中分离得到的。基于16S rRNA基因以及fusA、lepA、leuS、gyrB和ileS这五个管家基因的串联序列进行系统发育分析，结果表明菌株C1112(T)属于普罗威登斯菌属，与斯氏普罗威登斯菌DSM 4539(T)的相似度为91.75%。菌株C1112(T)与斯氏普罗威登斯菌KCTC 2568(T)之间的DNA-DNA杂交相关性为48.1%。此外，一些生化特性结果表明菌株C1112(T)与其系统发育关系最近的亲缘菌株不同。菌株C1112(T)的主要脂肪酸为C16:0、异-C15:0、C14:0和C17:0环丙烷，DNA G+C含量为41 mol%。基于基因型和表型方面的考虑，应将其分类为普罗威登斯菌属的一个新物种，为此提出泰国普罗威登斯菌（Providencia thailandensis sp. nov.）这一名称。模式菌株为C1112(T)（=KCTC 23281(T)=NBRC 106720(T)）。
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