黄白杆菌属，新属. 新种。从红树林沉积物中分离得到，建议设立黄白杆菌科，新科。
Bruguierivorax albus gen. nov. sp. nov. Isolated from Mangrove Sediment and Proposal of Bruguierivoracaceae fam. nov.
机构信息
Institute of Marine Drugs, School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, NO. 13 Wuhe Rood, Nanning, 530200, People's Republic of China.
出版信息
Curr Microbiol. 2021 Feb;78(2):856-866. doi: 10.1007/s00284-020-02311-w. Epub 2021 Jan 19.
A novel Gram-negative, motile, aerobic rod-shaped bacterium designated BGMRC 2031 was isolated from mangrove sediment collected from Guangxi Province, China. Optimal growth occurred at 28 °C and pH 7.0-8.0 in the presence of 1% (w/v) NaCl. Alignment based on 16S rRNA gene sequences indicated that strain BGMRC 2031 is most closely related to Sodalis praecaptivus HS1 (95.6%, sequence similarity), followed by Biostraticola tofi DSM 19580 (95.5%), Sodalis glossinidius DSM 16929 (95.4%), and Brenneria goodwinii FRB141 (94.9%) sequence similarity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BGMRC 2031 formed a distinct branch in a robust cluster and revealed that strain BGMRC 2031, genera Biostraticola and Sodalis, formed a novel family-level clade in the order Enterobacterales. The novel strain showed an average nucleotide similarity of 74.7%, 74.2%, and 73.1% for S. praecaptivus HS1, S. glossinidius DSM 16929, and B. tofi DSM 19580, respectively. The genomes of the BGMRC 2031 shared the presence of a riboflavin synthesis gene cluster. The menaquinones of strain BGMRC 2031 were MK-8 and Q-8, which were similar to those of genus Biostraticola. The major fatty acids (> 10%) were C (19.9%), summed feature 2 (iso-C and/or C 3-OH, 18.10%), summed feature 3 (C ω7c and/or C ω6c, 15.3%), C (13.9%), C cyclo (11.4%), and C (10.4%). The main polar lipids were phosphatidyl methylethanolamine, phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl inositol, one unidentified phospholipid, and one unknown polar lipid. The G+C content of strain BGMRC 2031 was 55.4%. Strain BGMRC 2031 could extend the mean lifespan and maximum lifespan of Caenorhabditis elegans by 4.5% and 12.5%, respectively. Overall, the results of this study indicate that BGMRC 2031 is a novel species in a new genus, for which the name Bruguierivorax albus gen. nov. sp. nov. is proposed, and the type of strain is designated as BGMRC 2031 (= NBRC 111907 = KCTC 52119). In addition, a novel family, Bruguierivoracaceae fam. nov., is proposed to accommodate the genera Bruguierivorax, Biostraticola, and Sodalis.
一株新的革兰氏阴性、运动、需氧杆状菌，命名为BGMRC 2031，从中国广西省红树林沉积物中分离得到。最佳生长条件为 28°C、pH 值 7.0-8.0、1%（w/v）NaCl。基于 16S rRNA 基因序列的比对表明，菌株 BGMRC 2031 与 Sodalis praecaptivus HS1（序列相似度 95.6%）最为密切相关，其次是 Biostraticola tofi DSM 19580（95.5%）、Sodalis glossinidius DSM 16929（95.4%）和 Brenneria goodwinii FRB141（94.9%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 BGMRC 2031 在一个稳健的聚类中形成了一个独特的分支，并表明菌株 BGMRC 2031、Biostraticola 和 Sodalis 属形成了肠杆菌目中新的家族水平分支。该新菌株与 Sodalis praecaptivus HS1、Sodalis glossinidius DSM 16929 和 B. tofi DSM 19580 的平均核苷酸相似性分别为 74.7%、74.2%和 73.1%。BGMRC 2031 的基因组存在一个核黄素合成基因簇。菌株 BGMRC 2031 的menaquinones 为 MK-8 和 Q-8，与 Biostraticola 属相似。主要脂肪酸（>10%）为 C（19.9%）、特征 2 总和（iso-C 和/或 C 3-OH，18.10%）、特征 3 总和（C ω7c 和/或 C ω6c，15.3%）、C（13.9%）、C 环（11.4%）和 C（10.4%）。主要极性脂质为磷脂酰甲醇乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂酰肌醇、一种未鉴定的磷脂和一种未知的极性脂质。BGMRC 2031 的 G+C 含量为 55.4%。菌株 BGMRC 2031 可分别将秀丽隐杆线虫的平均寿命和最大寿命延长 4.5%和 12.5%。总的来说，这项研究的结果表明，BGMRC 2031 是一种新属的新物种，建议命名为Bruguierivorax albus gen. nov. sp. nov.，其模式菌株被指定为 BGMRC 2031（=NBRC 111907=KCTC 52119）。此外，还提出了一个新的家族 Bruguierivoracaceae fam. nov.，以容纳 Bruguierivorax、Biostraticola 和 Sodalis 属。
Zotero 插件