评估 BD Bactec MGIT 320 系统检测分枝杆菌和结核分枝杆菌药物敏感性试验。
Evaluation of the BD Bactec MGIT 320 system for detection of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis.
机构信息
Microbial Diseases Laboratory, DCDC, CID, California Department of Public Health, Richmond, California, USA.
出版信息
J Clin Microbiol. 2013 Oct;51(10):3403-5. doi: 10.1128/JCM.01357-13. Epub 2013 Jul 17.
Abstract
Two different laboratories evaluated growth and detection of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis by the BD Bactec MGIT 320 using the BD Bactec MGIT 960 (BD Diagnostics, Sparks, MD) as a reference method. Out of 359 processed sputum specimens for detection of mycobacteria, 99.7% were in agreement between the MGIT 320 and MGIT 960. Streptomycin (STR), isoniazid (INH), rifampin (RIF), ethambutol (EMB) (collectively known as SIRE), and pyrazinamide (PZA) drug susceptibility testing was performed on 89 clinical strains, prepared from both liquid and solid inocula. The results of SIRE and PZA were 100% reproducible between the two instruments tested at both laboratories.
摘要
两个不同的实验室使用 BD Bactec MGIT 960（BD 诊断，斯帕克斯，MD）作为参考方法，评估了 BD Bactec MGIT 320 对分枝杆菌的生长和检测以及结核分枝杆菌的药物敏感性测试。在 359 份用于检测分枝杆菌的处理痰液标本中，MGIT 320 和 MGIT 960 的一致性为 99.7%。链霉素（STR）、异烟肼（INH）、利福平（RIF）、乙胺丁醇（EMB）（统称为 SIRE）和吡嗪酰胺（PZA）药物敏感性测试在 89 株来自液体和固体接种物的临床菌株上进行。在两个实验室中，两种仪器的 SIRE 和 PZA 结果 100%可重复。
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