使用MGIT 320系统检测结核分枝杆菌的时间与新疫苗临床前测试中的菌落计数相关。
Time to detection of Mycobacterium tuberculosis using the MGIT 320 system correlates with colony counting in preclinical testing of new vaccines.
作者信息
Kolibab K, Yang A, Parra M, Derrick S C, Morris S L
机构信息
Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland, USA.
出版信息
Clin Vaccine Immunol. 2014 Mar;21(3):453-5. doi: 10.1128/CVI.00742-13. Epub 2013 Dec 26.
Abstract
Clinical studies have suggested that the enumeration of mycobacteria by using automated liquid systems is a faster and simpler alternative to quantitative cultures. Here, we show that the time to detection of M. tuberculosis growth as measured with the MGIT 320 liquid culture system inversely correlates with CFU determinations from culture on solid media and that mycobacterial quantification using the MGIT system is faster and easier to perform than CFU plating.
摘要
临床研究表明，使用自动化液体系统对分枝杆菌进行计数是一种比定量培养更快、更简单的替代方法。在此，我们表明，用MGIT 320液体培养系统测定结核分枝杆菌生长的检测时间与固体培养基上培养的CFU测定值呈负相关，并且使用MGIT系统进行分枝杆菌定量比CFU平板接种更快且更容易操作。
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3
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