Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA.
J Biomed Opt. 2013 Jul;18(7):076015. doi: 10.1117/1.JBO.18.7.076015.
Confocal scanning laser ophthalmoscopy (cSLO) enables high-resolution and high-contrast imaging of the retina by employing spatial filtering for scattered light rejection. However, to obtain optimized image quality, one must design the cSLO around scanner technology limitations and minimize the effects of ocular aberrations and imaging artifacts. We describe a cSLO design methodology resulting in a simple, relatively inexpensive, and compact lens-based cSLO design optimized to balance resolution and throughput for a 20-deg field of view (FOV) with minimal imaging artifacts. We tested the imaging capabilities of our cSLO design with an experimental setup from which we obtained fast and high signal-to-noise ratio (SNR) retinal images. At lower FOVs, we were able to visualize parafoveal cone photoreceptors and nerve fiber bundles even without the use of adaptive optics. Through an experiment comparing our optimized cSLO design to a commercial cSLO system, we show that our design demonstrates a significant improvement in both image quality and resolution.
共聚焦扫描激光检眼镜（cSLO）通过对散射光进行空间滤波来实现视网膜的高分辨率和高对比度成像。然而，为了获得优化的图像质量，必须根据扫描仪技术的局限性来设计 cSLO，并最小化眼像差和成像伪影的影响。我们描述了一种 cSLO 设计方法，最终设计出一种简单、相对便宜且紧凑的基于透镜的 cSLO，该设计在最小化成像伪影的同时，优化了 20 度视场（FOV）的分辨率和吞吐量之间的平衡。我们使用实验设置测试了我们的 cSLO 设计的成像能力，从中获得了快速和高信噪比（SNR）的视网膜图像。在较低的 FOV 下，即使没有使用自适应光学，我们也能够可视化旁中心锥光感受器和神经纤维束。通过将我们优化的 cSLO 设计与商业 cSLO 系统进行比较的实验，我们表明我们的设计在图像质量和分辨率方面都有显著的提高。
