Dubra Alfredo, Sulai Yusufu, Norris Jennifer L, Cooper Robert F, Dubis Adam M, Williams David R, Carroll Joseph
Biomed Opt Express. 2011 Jul 1;2(7):1864-76. doi: 10.1364/BOE.2.001864. Epub 2011 Jun 8.
The rod photoreceptors are implicated in a number of devastating retinal diseases. However, routine imaging of these cells has remained elusive, even with the advent of adaptive optics imaging. Here, we present the first in vivo images of the contiguous rod photoreceptor mosaic in nine healthy human subjects. The images were collected with three different confocal adaptive optics scanning ophthalmoscopes at two different institutions, using 680 and 775 nm superluminescent diodes for illumination. Estimates of photoreceptor density and rod:cone ratios in the 5°-15° retinal eccentricity range are consistent with histological findings, confirming our ability to resolve the rod mosaic by averaging multiple registered images, without the need for additional image processing. In one subject, we were able to identify the emergence of the first rods at approximately 190 μm from the foveal center, in agreement with previous histological studies. The rod and cone photoreceptor mosaics appear in focus at different retinal depths, with the rod mosaic best focus (i.e., brightest and sharpest) being at least 10 μm shallower than the cones at retinal eccentricities larger than 8°. This study represents an important step in bringing high-resolution imaging to bear on the study of rod disorders.
视杆光感受器与多种严重的视网膜疾病有关。然而，即使自适应光学成像技术问世，对这些细胞的常规成像仍难以实现。在此，我们展示了九名健康人类受试者连续视杆光感受器镶嵌图的首张体内图像。这些图像是在两个不同机构使用三种不同的共焦自适应光学扫描检眼镜采集的，采用680纳米和775纳米的超发光二极管进行照明。在5°-15°视网膜偏心度范围内对视光感受器密度和视杆：视锥细胞比例的估计与组织学研究结果一致，证实了我们通过对多个配准图像进行平均来分辨视杆镶嵌图的能力，无需额外的图像处理。在一名受试者中，我们能够确定距中央凹中心约190微米处首批视杆细胞的出现，这与先前的组织学研究结果一致。视杆和视锥光感受器镶嵌图在不同的视网膜深度聚焦，在视网膜偏心度大于8°时，视杆镶嵌图的最佳聚焦（即最亮和最清晰）比视锥至少浅10微米。这项研究是将高分辨率成像应用于视杆疾病研究的重要一步。
