Casella Stefania, Fazio Francesco, Russo Carmelo, Giudice Elisabetta, Piccione Giuseppe
1Francesco Fazio, Department of Veterinary Science, Polo Universitario Annunziata, University of Messina, 98168, Messina, Italy.
J Vet Diagn Invest. 2013 Sep;25(5):577-80. doi: 10.1177/1040638713495851. Epub 2013 Jul 17.
Haptoglobin (Hp), serum amyloid A (SAA), C-reactive protein (CRP), and white blood cells (WBC) were assessed in 20 dogs divided into 2 groups. The dogs of group A were not subjected to hunting exercise (control group), while the dogs of group B were subjected to hunting exercise for 3 hr (experimental group). Blood samples were collected from each animal before hunting (T0), immediately after 3 hr of hunting (T1), and after 1 hr of recovery (T2). The general linear model (GLM) repeated measures procedure showed a significant difference between the 2 groups (P < 0.0001) and a significant rise (P < 0.0001) in concentration of Hp, SAA, and CRP after hunting exercise, with a consequent decline during recovery period in group B. These parameters could be considered valid and easily obtainable biomarkers in relation to hunting stress in dogs. Additional studies will continue to elucidate the magnitude and the time of response of other acute phase proteins.
对20只狗进行分组，评估其触珠蛋白（Hp）、血清淀粉样蛋白A（SAA）、C反应蛋白（CRP）和白细胞（WBC）。A组犬不进行狩猎活动（对照组），而B组犬进行3小时的狩猎活动（实验组）。在狩猎前（T0）、狩猎3小时后立即（T1）以及恢复1小时后（T2）从每只动物采集血样。一般线性模型（GLM）重复测量程序显示两组之间存在显著差异（P < 0.0001），并且狩猎活动后Hp、SAA和CRP浓度显著升高（P < 0.0001），随后B组在恢复期浓度下降。这些参数可被视为与犬狩猎应激相关的有效且易于获得的生物标志物。进一步的研究将继续阐明其他急性期蛋白的反应程度和时间。
