Yoon Ji-Seon, Yu DoHyeon, Park Jinho
College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.
Front Vet Sci. 2021 Mar 1;8:626540. doi: 10.3389/fvets.2021.626540. eCollection 2021.
Serum proteins are involved in the regulation of inflammation, and therefore, serum protein profiling provides important insights in diverse inflammatory reactions. Accordingly, concentrations of single APPs, such as the C-reactive protein (CRP), serum amyloid A (SAA), and haptoglobin (Hp), have been described as indicators of inflammatory response in canine pyometra. However, there is little information regarding the overall serum protein profile obtained by SPE in canine pyometra. The present study was thus aimed to identify changes in the serum protein profile to monitor inflammation in dogs with pyometra using serum protein electrophoresis (SPE), in addition to the analysis of the concentration of single acute phase proteins (APPs). By SPE analysis, decreased levels of albumin and elevated levels of α2-globulin and β-globulin were noted in dogs with pyometra. In addition, the concentration of APPs, including the C-reactive protein (CRP), serum amyloid A (SAA), and haptoglobin (Hp), were also elevated in dogs with pyometra. The present study provides fundamental data for inflammatory indicators of canine pyometra.
血清蛋白参与炎症调节，因此，血清蛋白谱分析为多种炎症反应提供了重要见解。相应地，单一急性时相蛋白（APPs）的浓度，如C反应蛋白（CRP）、血清淀粉样蛋白A（SAA）和触珠蛋白（Hp），已被描述为犬子宫蓄脓炎症反应的指标。然而，关于犬子宫蓄脓通过血清蛋白电泳（SPE）获得的整体血清蛋白谱的信息很少。因此，本研究旨在除分析单一急性期蛋白（APPs）浓度外，通过血清蛋白电泳（SPE）鉴定血清蛋白谱的变化，以监测患有子宫蓄脓的犬的炎症。通过SPE分析，发现子宫蓄脓犬的白蛋白水平降低，α2球蛋白和β球蛋白水平升高。此外，子宫蓄脓犬中包括C反应蛋白（CRP）、血清淀粉样蛋白A（SAA）和触珠蛋白（Hp）在内的APPs浓度也升高。本研究为犬子宫蓄脓的炎症指标提供了基础数据。
