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不同产地东北鹿蹄草中活性成分的变化及抗氧化活性。

Variation of active constituents and antioxidant activity in pyrola [P. incarnata Fisch.] from different sites in Northeast China.

机构信息

State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China.

出版信息

Food Chem. 2013 Dec 1;141(3):2213-9. doi: 10.1016/j.foodchem.2013.05.045. Epub 2013 May 21.

DOI:10.1016/j.foodchem.2013.05.045
PMID:23870950
Abstract

The variation of antioxidant activity and active components in pyrola [Passiflora incarnata Fisch.] from eight sites in Northeast China were investigated. Total phenolic and flavonoid contents were determined and varied within the range of 39.66-181.48 mg/g and 2.47-22.11 mg/g, respectively. Antioxidant activities were determined by scavenging activity against DPPH and ABTS, by a reducing power test and by a β-carotene-linoleic acid bleaching test. The IC50 of Tahe samples determined by the DPPH test was 0.106±0.006 mg/mL which was very close to that of Vc (0.076±0.004 mg/mL). The Tahe samples had good antioxidant activity. Principal component activity analysis indicated that the Tahe samples of P. incarnata had the highest potential antioxidant properties, and may be a valuable antioxidant natural resource in the northeast of China.

摘要

对中国东北 8 个产地的鹿药[西番莲(Passiflora incarnata Fisch.)]的抗氧化活性和活性成分的变化进行了研究。总酚和类黄酮含量的测定范围分别为 39.66-181.48mg/g 和 2.47-22.11mg/g。抗氧化活性通过 DPPH 和 ABTS 清除活性、还原能力测试和β-胡萝卜素-亚油酸漂白测试来测定。用 DPPH 法测定的 Tahe 样品的 IC50 为 0.106±0.006mg/mL,非常接近 Vc(0.076±0.004mg/mL)。Tahe 样品具有良好的抗氧化活性。主成分活性分析表明,Tahe 产的西番莲具有最高的潜在抗氧化特性,可能是中国东北地区有价值的抗氧化天然资源。

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