Zhou Mingming, Lin Yuan, Fang Shengzuo, Liu Yang, Shang Xulan
College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, China.
PeerJ. 2019 Feb 19;7:e6492. doi: 10.7717/peerj.6492. eCollection 2019.
(Batal) Iljinskaja is a multiple function tree species, but its main utilization is for the harvesting of its leaves as materials for tea production and recently as ingredients for the food industry. In this study aqueous extracts of leaves collected from 21 natural populations were evaluated for their phytochemical content and antioxidant activity. The content of water-soluble polysaccharide, total flavonoid and total polyphenol varied from 66.05 to 153.32 mg/g, 9.01 to 19.65 mg/g and 20.80 to 52.69 mg/g, respectively. Quercetin-3--glucuronide, aemferol-3--glucuronide and 3--caffeoylquinic acid were the major phenolic components in aqueous extracts of leaves. Both redundancy analysis and Pearson's correlation analysis indicated that water-soluble polysaccharide, total polyphenol, total flavonoid, quercetin-3--glucuronide, 3--caffeoylquinic acid and 4--caffeoylquinic acid were significantly correlated with antioxidant activity, but total polyphenol showed the greatest contribution to antioxidant capacity. The antioxidant activity of the 21 populations was classified into six distinct groups based on the squared Euclidean distance. These results would provide a theoretical basis for obtaining the greatest yield of targeted antioxidant phytochemicals of leaves for tea and food ingredient production.
（巴塔尔）伊尔金斯卡亚树是一种具有多种功能的树种，但其主要用途是采摘树叶作为茶叶生产的原料，最近也用作食品工业的原料。在本研究中，对从21个自然种群采集的树叶水提取物的植物化学成分和抗氧化活性进行了评估。水溶性多糖、总黄酮和总多酚的含量分别在66.05至153.32毫克/克、9.01至19.65毫克/克和20.80至52.69毫克/克之间。槲皮素-3-葡萄糖醛酸苷、山奈酚-3-葡萄糖醛酸苷和3-咖啡酰奎尼酸是树叶水提取物中的主要酚类成分。冗余分析和皮尔逊相关分析均表明，水溶性多糖、总多酚、总黄酮、槲皮素-3-葡萄糖醛酸苷、3-咖啡酰奎尼酸和4-咖啡酰奎尼酸与抗氧化活性显著相关，但总多酚对抗氧化能力的贡献最大。根据平方欧氏距离，将21个种群的抗氧化活性分为六个不同的组。这些结果将为获取用于茶叶和食品配料生产的树叶中目标抗氧化植物化学成分的最大产量提供理论依据。
