Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes.
Appl Environ Microbiol. 2013 Oct;79(19):5927-35. doi: 10.1128/AEM.02251-13. Epub 2013 Jul 19.
Hydrocarbon-degrading bacterial communities from freshwater, marine, and hypersaline Brazilian aquatic ecosystems (with water salinities corresponding to 0.2%, 4%, and 5%, respectively) were enriched with different hydrocarbons (heptadecane, naphthalene, or crude oil). Changes within the different microcosms of bacterial communities were analyzed using cultivation approaches and molecular methods (DNA and RNA extraction, followed by genetic fingerprinting and analyses of clone libraries based on the 16S rRNA-coding gene). A redundancy analysis (RDA) of the genetic fingerprint data and a principal component analysis (PCA) of the clone libraries revealed hydrocarbon-enriched bacterial communities specific for each ecosystem studied. However, within the same ecosystem, different bacterial communities were selected according to the petroleum hydrocarbon used. In general, the results demonstrated that Acinetobacter and Cloacibacterium were the dominant genera in freshwater microcosms; the Oceanospirillales order and the Marinobacter, Pseudomonas, and Cycloclasticus genera predominated in marine microcosms; and the Oceanospirillales order and the Marinobacter genus were selected in the different hydrocarbon-containing microcosms in hypersaline water. Determination of total petroleum hydrocarbons (TPHs) in all microcosms after 32 days of incubation showed a decrease in the hydrocarbon concentration compared to that for the controls. A total of 50 (41.3%) isolates from the different hydrocarbon-contaminated microcosms were associated with the dominant operational taxonomic units (OTUs) obtained from the clone libraries, and their growth in the hydrocarbon contaminating the microcosm from which they were isolated as the sole carbon source was observed. These data provide insight into the general response of bacterial communities from freshwater, marine, and hypersaline aquatic ecosystems to petroleum hydrocarbon contamination.
从巴西淡水、海洋和高盐度水生生态系统（水的盐度分别对应于 0.2%、4%和 5%）中富集了不同烃类（十七烷、萘或原油）的降解烃类的细菌群落。使用培养方法和分子方法（DNA 和 RNA 提取，然后进行基于 16S rRNA 编码基因的遗传指纹分析和克隆文库分析）分析了不同微生物群落的变化。遗传指纹数据的冗余分析（RDA）和克隆文库的主成分分析（PCA）显示，每种研究生态系统都具有特定的烃富集细菌群落。然而，在相同的生态系统中，根据使用的石油烃类选择了不同的细菌群落。一般来说，结果表明，不动杆菌属和 Cloacibacterium 是淡水微生物群落中的优势属；海洋螺旋体目和 Marinobacter、Pseudomonas 和 Cycloclasticus 属在海洋微生物群落中占优势；海洋螺旋体目和 Marinobacter 属在高盐度水中的不同含烃微生物群落中被选择。在 32 天的孵育后，所有微宇宙中的总石油烃（TPH）的测定表明，与对照相比，烃浓度降低。从不同烃污染微宇宙中分离的总共 50 个（41.3%）分离株与从克隆文库获得的主要操作分类单元（OTU）相关，并且观察到它们在作为唯一碳源污染微宇宙的烃中的生长。这些数据提供了对淡水、海洋和高盐度水生生态系统细菌群落对石油烃污染的一般反应的深入了解。
