Cancer Genetics Laboratory, Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
PLoS One. 2013 Jul 17;8(7):e69607. doi: 10.1371/journal.pone.0069607. Print 2013.
Minichromosome Maintenance (MCM) proteins play important roles in cell cycle progression by mediating DNA replication initiation and elongation. Among 10 MCM homologues MCM 2-7 form a hexamer and assemble to the pre-replication complex acting as replication licensing factors. Binding and function of MCM2-7 to pre-replication complex is regulated by MCM10 mediated binding of RECQL4 with MCM2-7. The purpose of this study is to explore the role of MCMs in cervical cancer and their correlation with the clinical parameters of cervical cancer. We have investigated sixty primary cervical cancer tissue samples, eight cervical cancer cell lines and thirty hysterectomised normal cervical tissue. The expression profiling of MCMs was done using semi-quantitative RT-PCR, immunoblotting and immunohistochemistry. MCM2, 4, 5, 6, 7, 10 and RECQL4 are significantly over-expressed in cervical cancer. Among these, MCM4, 6 and 10 show increased frequency of over expression along with advancement of tumor stages. MCM4, 5 and 6 also show differential expression in different types of lesion, while MCM2 and MCM10 are over expressed in cervical cancer irrespective of clinico-pathological parameters. Our data indicates the role of MCM4, MCM5, MCM6, MCM10 and RECQL4 in the progression of cervical cancer.
微小染色体维持 (MCM) 蛋白在细胞周期进程中发挥重要作用，通过介导 DNA 复制起始和延伸。在 10 个 MCM 同源物中，MCM2-7 形成六聚体并组装到前复制复合物中，作为复制许可因子。MCM10 介导 RECQL4 与 MCM2-7 的结合和功能调节 MCM2-7 与前复制复合物的结合。本研究旨在探讨 MCM 在宫颈癌中的作用及其与宫颈癌临床参数的相关性。我们研究了六十例原发性宫颈癌组织样本、八株宫颈癌细胞系和三十例子宫切除的正常宫颈组织。使用半定量 RT-PCR、免疫印迹和免疫组织化学方法研究了 MCM 的表达谱。MCM2、4、5、6、7、10 和 RECQL4 在宫颈癌中明显过表达。其中，MCM4、6 和 10 的过表达频率随着肿瘤分期的进展而增加。MCM4、5 和 6 在不同类型的病变中也表现出差异表达，而 MCM2 和 MCM10 则不论临床病理参数如何都在宫颈癌中过表达。我们的数据表明 MCM4、MCM5、MCM6、MCM10 和 RECQL4 在宫颈癌的进展中发挥作用。
