Vincent Center for Reproductive Biology, Massachusetts General Hospital, and Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, Massachusetts.
Fertil Steril. 2013 Nov;100(5):1451-8. doi: 10.1016/j.fertnstert.2013.06.036. Epub 2013 Jul 19.
Perform gene expression profiling of adult mouse ovary-derived oogonial stem cells (OSCs).
Experimental animal study.
Research laboratory.
ANIMAL(S): Adult C57BL/6 female mice.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Gene expression profiles were compared between freshly isolated and cultured OSCs, as well as between OSCs and embryonic stem cells (ESCs), fetal primordial germ cells (PGCs), and spermatogonial stem cells (SSCs); OSC yield from ovaries versus meiotic gene activation during the estrous cycle was determined.
RESULT(S): Freshly isolated OSCs, PGCs, and SSCs exhibited distinct gene expression profiles. Cultured OSCs maintained their germline gene expression pattern but gained expression of pluripotency markers found in PGCs and ESCs. Cultured OSCs also expressed the meiotic marker, stimulated by retinoic acid gene 8 (Stra8). In vivo, OSC yield was higher from luteal versus follicular phase ovaries, and this was inversely related to Stra8 expression.
CONCLUSION(S): Freshly isolated OSCs exhibit a germline gene expression profile that overlaps with, but is distinct from, that of PGCs and SSCs. After in vitro expansion, OSCs activate expression of pluripotency genes found in freshly isolated PGCs. In vivo, OSC numbers in the ovaries fluctuate during the estrous cycle, with the highest numbers noted during the luteal phase. This is followed by activation of Stra8 expression during the follicular phase, which may signify a wave of neo-oogenesis to partially offset follicular loss through atresia and ovulation in the prior cycle.
对成年小鼠卵巢来源的卵原干细胞（OSCs）进行基因表达谱分析。
实验动物研究。
研究实验室。
成年 C57BL/6 雌性小鼠。
无。
比较新鲜分离和培养的 OSCs 之间，以及 OSCs 与胚胎干细胞（ESCs）、胎儿原始生殖细胞（PGCs）和精原干细胞（SSCs）之间的基因表达谱；确定卵巢中 OSCs 的产量与发情周期中减数分裂基因激活之间的关系。
新鲜分离的 OSCs、PGCs 和 SSCs 表现出明显不同的基因表达谱。培养的 OSCs 保持其生殖系基因表达模式，但获得了在 PGCs 和 ESCs 中发现的多能性标记物的表达。培养的 OSCs 还表达了减数分裂标记物，即视黄酸基因 8（Stra8）刺激。在体内，黄体期卵巢中的 OSCs 产量高于卵泡期，这与 Stra8 表达呈负相关。
新鲜分离的 OSCs 表现出与 PGCs 和 SSCs 重叠但又不同的生殖系基因表达谱。在体外扩增后，OSCs 激活了在新鲜分离的 PGCs 中发现的多能性基因的表达。在体内，发情周期中卵巢中的 OSCs 数量波动，黄体期数量最高。随后在卵泡期激活 Stra8 表达，这可能标志着新的卵发生波，以部分抵消前一周期中通过闭锁和排卵导致的卵泡损失。
