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基于现场的流式细胞术用于体外鉴定间日疟原虫和恶性疟原虫的抗疟药物敏感性。

Field-based flow cytometry for ex vivo characterization of Plasmodium vivax and P. falciparum antimalarial sensitivity.

机构信息

Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.

出版信息

Antimicrob Agents Chemother. 2013 Oct;57(10):5170-4. doi: 10.1128/AAC.00682-13. Epub 2013 Jul 22.

DOI:10.1128/AAC.00682-13
PMID:23877705
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3811473/
Abstract

Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated Plasmodium vivax and Plasmodium falciparum.

摘要

体外抗疟原虫敏感性检测在人类疟原虫中主要依赖于裂殖体成熟的显微镜检测。虽然这种显微镜方法很敏感，但它存在精度差和繁琐的问题。最近便携式、低成本的细胞仪的发展使我们能够开发和验证一种简单、适用于现场的方案，该方案使用 SYBR 绿和二氢乙锭，用于准确和客观地测定新鲜分离的间日疟原虫和恶性疟原虫对抗疟药物的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3756/3811473/53eed2d92d31/zac0101322090002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3756/3811473/efd8bc760621/zac0101322090001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3756/3811473/53eed2d92d31/zac0101322090002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3756/3811473/efd8bc760621/zac0101322090001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3756/3811473/53eed2d92d31/zac0101322090002.jpg

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