Department of Life Science and Research Center for Biomolecules and Biosystems, Chung-Ang University, 84, HeukSeok-Ro, Dongjak-Gu, Seoul, 156-756, Republic of Korea.
Antonie Van Leeuwenhoek. 2013 Mar;103(3):503-12. doi: 10.1007/s10482-012-9832-x. Epub 2012 Oct 25.
Two moderately halophilic, facultatively aerobic, motile bacteria with flagella, designated strains 10-C-3(T) and 30-C-3, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of the strains were observed to be ovoid-rods showing catalase- and oxidase-positive reactions and production of creamy-pink pigments. Growth of strain 10-C-3(T) was observed at 15-35 °C (optimum, 25-30 °C), at pH 5.5-9.0 (optimum, pH 7.0-7.5), and in the presence of 3-15 % (w/v) salts (optimum: 5-10 %). The two strains were found to contain C(18:1) ω7c, C(16:0), summed feature 3 (as defined by the MIDI system, comprising C(16:1) ω7c and/or C(16:1) ω6c), and C(12:0) 3-OH as the major cellular fatty acids. The G+C contents of the genomic DNA of strains 10-C-3(T) and 30-C-3 were determined to be 63.2 and 63.1 mol%, respectively and the respiratory quinone detected was ubiquinone 9 (Q-9) only. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 10-C-3(T)and 30-C-3 formed a distinct phyletic lineage within the genus Halomonas and are most closely related to Halomonas fontilapidosi 5CR(T) with 95.2 % of 16S rRNA sequence similarity. Strains 10-C-3(T)and 30-C-3 shared 99.2 % of 16S rRNA gene sequence similarity and their DNA-DNA relatedness value was 96.6 ± 0.9 %. On the basis of phenotypic, chemotaxonomic and molecular features, strains 10-C-3(T)and 30-C-3 represent a novel species of the genus Halomonas, for which the name Halomonas cibimaris sp. nov. is proposed. The type strain is 10-C-3(T) (= KACC 14932(T) = JCM 16914(T)).
两株中度嗜盐、兼性需氧、能运动的细菌，带有鞭毛，分别命名为 10-C-3(T) 和 30-C-3 株，从韩国传统发酵海鲜 jeotgal 中分离得到。观察到菌株的细胞呈卵形-棒状，呈过氧化氢酶和氧化酶阳性反应，并产生乳脂色颜料。菌株 10-C-3(T) 的生长温度为 15-35°C（最佳温度为 25-30°C），pH 值为 5.5-9.0（最佳 pH 值为 7.0-7.5），盐度为 3-15%（w/v）（最佳为 5-10%）。发现这两株菌含有 C(18:1) ω7c、C(16:0)、总和特征 3（由 MIDI 系统定义，包括 C(16:1) ω7c 和/或 C(16:1) ω6c）和 C(12:0) 3-OH 作为主要细胞脂肪酸。菌株 10-C-3(T) 和 30-C-3 的基因组 DNA 的 G+C 含量分别确定为 63.2 和 63.1mol%，检测到的呼吸醌仅为泛醌 9（Q-9）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 10-C-3(T)和 30-C-3 在盐单胞菌属中形成一个独特的进化分支，与 Halomonas fontilapidosi 5CR(T) 的 16S rRNA 序列相似性最高，为 95.2%。菌株 10-C-3(T)和 30-C-3 的 16S rRNA 基因序列相似性为 99.2%，其 DNA-DNA 相关值为 96.6±0.9%。基于表型、化学分类和分子特征，菌株 10-C-3(T)和 30-C-3 代表盐单胞菌属的一个新种，建议将其命名为 Halomonas cibimaris sp. nov.。模式菌株为 10-C-3(T)（=KACC 14932(T)=JCM 16914(T)）。
