利用磁阱对解旋酶进行的机理研究。
A mechanistic study of helicases with magnetic traps.
作者信息
Hodeib Samar, Raj Saurabh, Manosas Maria, Zhang Weiting, Bagchi Debjani, Ducos Bertrand, Fiorini Francesca, Kanaan Joanne, Le Hir Hervé, Allemand Jean-François, Bensimon David, Croquette Vincent
机构信息
Laboratoire de physique statistique, Département de physique de l'ENS, École normale supérieure, PSL Research University, Université Paris Diderot, Sorbonne Paris Cité, Sorbonne Universités, UPMC Univ. Paris 06, CNRS, 75005, Paris, France.
Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Département de biologie, École normale supérieure, CNRS, INSERM, PSL Research University, 75005, Paris, France.
出版信息
Protein Sci. 2017 Jul;26(7):1314-1336. doi: 10.1002/pro.3187. Epub 2017 Jun 13.
Helicases are a broad family of enzymes that separate nucleic acid double strand structures (DNA/DNA, DNA/RNA, or RNA/RNA) and thus are essential to DNA replication and the maintenance of nucleic acid integrity. We review the picture that has emerged from single molecule studies of the mechanisms of DNA and RNA helicases and their interactions with other proteins. Many features have been uncovered by these studies that were obscured by bulk studies, such as DNA strands switching, mechanical (rather than biochemical) coupling between helicases and polymerases, helicase-induced re-hybridization and stalled fork rescue.
解旋酶是一类广泛的酶，可分离核酸双链结构（DNA/DNA、DNA/RNA或RNA/RNA），因此对DNA复制和核酸完整性的维持至关重要。我们综述了从单分子研究中得出的关于DNA和RNA解旋酶机制及其与其他蛋白质相互作用的情况。这些研究揭示了许多在整体研究中被掩盖的特征，例如DNA链切换、解旋酶与聚合酶之间的机械（而非生化）偶联、解旋酶诱导的重新杂交以及停滞叉拯救。
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