Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand,
Mycotoxin Res. 2013 Nov;29(4):255-60. doi: 10.1007/s12550-013-0173-z. Epub 2013 Jul 28.
Dried Distiller's Grains with Solubles (DDGS), a by-product of bio-ethanol production from maize and other cereals, is increasingly used as a feed additive. In this study, five Fusarium toxins, including fumonisin B1 (FB1), fumonisin B2 (FB2), deoxynivalenol (DON), zearalenone (ZEN) and beauvericin (BEA) were quantified by LC-MS/MS in 59 corn-DDGS samples. In addition, the fumonisin level in 30 randomly selected-samples was compared using an ELISA detection technique. No sample was free from mycotoxin contamination, and 50.8 % of the samples were co-contaminated with all five mycotoxins. Moreover, toxin levels were generally high, with mean levels of 9 mg kg(-1) FB1, 6 mg kg(-1) FB2, 1.2 mg kg(-1) DON, 0.9 mg kg(-1) ZEN, and 0.35 mg kg(-1) BEA. Maximum levels for FB1 (143 mg kg(-1)) and FB2 (125 mg kg(-1)) are of acute toxicological relevance. The ELISA method had a tendency to underestimate the fumonisin content when compared with LC-MS/MS. Finally, this is the first reported beauvericin contamination in corn-DDGS.
干燥的酒糟可溶物（DDGS）是从玉米和其他谷物生产生物乙醇的副产品，越来越多地用作饲料添加剂。在这项研究中，采用 LC-MS/MS 定量检测了 59 个玉米-DDGS 样品中的 5 种镰刀菌毒素，包括伏马菌素 B1（FB1）、伏马菌素 B2（FB2）、脱氧雪腐镰刀菌烯醇（DON）、玉米赤霉烯酮（ZEN）和 beauvericin（BEA）。此外，还使用 ELISA 检测技术比较了 30 个随机选择的样品中的 fumonisin 水平。没有一个样品没有霉菌毒素污染，50.8%的样品同时受到所有 5 种霉菌毒素的污染。此外，毒素水平普遍较高，FB1 的平均水平为 9mgkg(-1)，FB2 为 6mgkg(-1)，DON 为 1.2mgkg(-1)，ZEN 为 0.9mgkg(-1)，BEA 为 0.35mgkg(-1)。FB1（143mgkg(-1)）和 FB2（125mgkg(-1)）的最大水平具有急性毒理学相关性。与 LC-MS/MS 相比，ELISA 方法检测 fumonisin 含量时存在低估的趋势。最后，这是首次报道玉米-DDGS 中 beauvericin 的污染。
